1. Separation technology Ultrahigh-pressure chromatography technique development High-efficiency LC (e.g., with separation peak capacities >1000) High-speed LC (e.g., for separations within 5 minutes) Ultrasensitive LC (e.g., with 15-20 um i.d. capillary columns) High-resolution capillary zone electrophoresis and isoelectric focusing of peptides, proteins, protein complexes, and intact cells Supercritical fluid chromatography and gas chromatography2 Mass spectrometry High-resolution tandem mass spectrometry for sequencing of polypeptides and their modifications High-resolution single-stage mass spectrometry for high-throughput imaging of proteomes3. Proteomics Single-dimension proteomics Large dynamic range two-dimensional proteomics Nanoproteomics and single cell proteomics Ultrahigh-throughput proteomics Cancer degradomic-peptidomics