Sequence 8-oxo-dG by NGS and perform bioinformatics by R, python and Linux pipelines.Investigate the functions of promoter G-quadruplexes in vitro and in vivo (E.coli, HeLa and MEF cells) via luciferase reporter assays.• Python: Numpy, Scipy, Pandas, Re, Ipython, Matplotlib, Seaborn• R: Tidyr, ggplot2, stringr, readr, dplyr, reshape2, Bioconductor • LINUX: AWK, Sed, vi• Aligners: NovoAlign, Bowtie, Tophat2, cuffdiff2• Peakcaller and motif analysis: MACS2, MEME• DNA Sequencing: Bedtools, Samtools, LoFreq, GATK, Varscan2, Mutect, FreeBayes• Databases: Biomart, Ensembl, SRA, UCSC genome browser, GEO, TCGA

Study DNA secondary structures such as DNA hairpins, and DNA modifications such as 5-methyl-C by protein nanopore (third generation DNA sequencing technique), HPLC, Uv/vis, CD, and MS. Internal hairpins and fishhook hairpins were found to have different unzipping mechanisms and locations. Explore the latch zone of alpha-hemolysin with a group fishhook hairpin structures. Besides abasic site, current differences between native base pairs were detected.Investigate the interactions of DNA hairpin with groove binders such as polyamines by protein nanopore. The binding of Spm and Spd were so strong that the hairpins will not unzip, while Put does not have an effect on the unzipping of hairpins.Demonstrated the kinetics of different length of CAG repeats are different even though they have similar thermodynamic stability.

Taught General Chemistry Lab

Taught General Chemistry for Grade 1 in NO.1 High School in Kaifeng, HenanDeveloped teaching materials Inspired students to find their own learning style

Designed, synthesized, and characterized iron titanate catalysts for the selective catalytic reduction (SCR) of NO with NH3 by FT-IR, BET, XRD, and etc.Synthesized manganese-substitued iron titanate catalysts for the SCR of NO and NH3.Determined the NO conversion over different catalysts by FT-IR.Characterized the pore size, pore volume, pore diameter and structure of the catalysts by BET and XANES.Performed H2-TPR (temperature programmed reduction), NH3-TPD, AND NO-TPD (temperature programmed desorption)using quadrupole mass spectrometer.

Developed methods to monitor volatile organic compounds (VOCs) in Children's exhalation by GC-MS in Prof. Zhao's groupSet up and troubleshoot instrumentAnalytical method development and transfer