Graduate Student Researcher
➢ Multiphoton excitation microscopy in pattern scanning• Designed and built a high-speed (~200Hz) two-photon excitation scanning microscopy from scratch, and achieved a more accurate neuron activity analysis with a lower power deposit to sample plane• Conducted in-vivo test on mouse brain and post-processed neuronal signals with denoising and demixing• Modeled and verified the temporal focusing properties on diffraction components with focused beam and large Airy pattern from MATLAB and Lumerical FDTD with phantom tests experiments• Designed the large NA large FOV objective lens and optimized the aberrations in Zemax ➢ V-Shape PSF with Double Axicon Lens in Wide Field Microscopy• Developed a new type of PSF for wide-field microscopy with 3D resolving power over an extended depth of field which enables the volumetric imaging at a single-shot.• Designed and simulated the PSF technique for a 10x microscope with CAD and NSC Zemax simulation• Reconstructed the images at different depths over a 350um range through a deep neural network