Intralytix is phage biotech company with +20 years of experience developing multiple phage-based products that were mostly approved by the USDA.While working there, some of my biggest projects and tasks included:-characterizing, optimizing the production of multiple Kayviruses (a genus of S. aureus phages) and validated that they were incapable of viral transduction-compared the lytic spectrum and plating efficiency of 7 EcoActive (adhesive-invasive E. coli) phages that were… Show more Intralytix is phage biotech company with +20 years of experience developing multiple phage-based products that were mostly approved by the USDA.While working there, some of my biggest projects and tasks included:-characterizing, optimizing the production of multiple Kayviruses (a genus of S. aureus phages) and validated that they were incapable of viral transduction-compared the lytic spectrum and plating efficiency of 7 EcoActive (adhesive-invasive E. coli) phages that were originally produced on pathogenic strains and switched to “safer” host strains-attempting to isolate obligately lytic Group B Streptococcus Phages-achieving optimal growth conditions to produce large volumes of high titer, buffer-exchanged Vibrio corallyticus phage stocks used in oyster larval studies-being the bacterial and lyophilized phage strain librarian-pulled phage and bacterial characterization information from disconnected reports and manuscripts and centralized the information on Intralytix’s lab inventory/info management system (LIMS)-Offered feedback and recommendations to improve implementation of previously-made features and requested useful novel features to add to their LIMS Show less

Worked at a phage therapy biotech company and helped assess the efficacy of obligately lytic E. coli & S. aureus phages to treat Bovine mastitis, ETEC and STEC bacterial infections.Since joining I helped: -build and assess EpiBiome's library of obligately lytic phages-developed and performed column chromatography-based purification and QC ELISAs and experiments to create therapeutic cocktails for multiple animal studies that met viable titer and purity specifications-consult… Show more Worked at a phage therapy biotech company and helped assess the efficacy of obligately lytic E. coli & S. aureus phages to treat Bovine mastitis, ETEC and STEC bacterial infections.Since joining I helped: -build and assess EpiBiome's library of obligately lytic phages-developed and performed column chromatography-based purification and QC ELISAs and experiments to create therapeutic cocktails for multiple animal studies that met viable titer and purity specifications-consult, troubleshoot, offer feedback, draft SOPs and perform automated high throughput phage discovery experiments-participated in two research grants, both phage biology related Show less

2010 - 2011 I worked on exploring and developing bacteriophage cocktails in vitro that could be used to control E. coli O157:H7. I learned how to generate stable, single-phage resistant mutants (used for enumerating a titer of a single phage type), run detailed phage infections that showed burst size, the eclipse and latent periods.2012 - 2014 I had been working on isolating and characterizing lytic phages that infect Yersinia ruckeri using PFGE, host ranges and studying virus-host… Show more 2010 - 2011 I worked on exploring and developing bacteriophage cocktails in vitro that could be used to control E. coli O157:H7. I learned how to generate stable, single-phage resistant mutants (used for enumerating a titer of a single phage type), run detailed phage infections that showed burst size, the eclipse and latent periods.2012 - 2014 I had been working on isolating and characterizing lytic phages that infect Yersinia ruckeri using PFGE, host ranges and studying virus-host interactions as a side project. My main project in this time period has been studying virus metabolism and progeny production in stationary phase hosts using E. coli and bacteriophage T4 along with T4 amber, deletion and recombinant mutants. Show less

I was a coordinator for the 20th Biennial Evergreen International Phage Conference (for more information about the conference please visit www.evergreen.edu/phage). My roles included setting up electronic registration, handling money and accounts, managing information of applicants, chauffeuring guests and setting up and cleaning up for poster sessions and dinner events.