Research Assistant
Stony Brook, Ny, Us
Joined Reich Lab to study Pancreatic Cancer focusing on the YAP1 in combination with kRas proto-oncogenes in the Microbiology and Immunology Department.Conducted mammalian tissue culture work by maintaining, splitting, freezing, thawing, and counting cells. Used HeLa, 293T, and B6 cell lines.Performed CRISPR/Cas9 protocol to "knock out" YAP1 gene by bacterial cloning, PCR, Plasmid Design, DNA sequencing, Transfection of Lentivirus, Western Blots on gene product of cells, and injecting immunocompromised mice to check for tumor growth.Utilized Microsoft Excel to analyze protein concentrations, viral titers, tumor growth in mice over time.