Zaker Khan Email & Phone Number

Stockbridge, Ga, Us

Stockbridge, Ga, Us

• Currently working as a process engineer in the improve plant department to further develop and modify the following processes: Fermentation, Aqueous Extraction, Hexane Extraction, Spray Drying, and Refinery• Developing necessary documentation including but not limited to: Management of Change (MOC) forms, Process Evaluation Plan (PEP) reports, Scope of work Installation/Cost analysis documents, and Capital Expenditure forms, to outline project actions and implementation protocols.• Creating Process Flow Diagrams and Red Line P&ID’s to update HAZOP’s and show the installation of new equipment and/or piping.• Traveling domestically/international to other DSM sites over the world to attend training sessions and observe lab and pilot scaling facilities.• Working with: engineers, operators, laboratory technicians, contractors, and other workers at the site to communicate effectively with all personnel making sure deadlines are met and everyone is aware of all practices in place.• Working with the sites process safety team to create chemical hazard assessments (CHA) for all product strains of DHA and ARA.• Leader of the employee relations committee team working with employees throughout the site to help with: employee engagement, completing company surveys, and expressing personal opinions about job duties.

Cardiff, Wales, Gb

• Worked the night shift under minimal supervision to enhance the upstream and downstream processing of various kinds of Bacillus strains.• Performed dry/batch sterilization on bioreactors of four different sizes (500L, 2000L, 4000L, and 30,000L) to ensure the vessels are free of microbial contamination.• Entered confined space vessels wearing Fall Protection Harnesses and checking atmospheric conditions in regulation to Occupational Safety and Health Administration (OSHA), to install spray balls and inspect the vessels for any loose piping prior to Clean-In-Place (CIP).• Performed CIP on all necessary equipment involved in bacteria production using: caustic soda, phosphoric acid, etc. in order to remove product residue and maintain an aseptic work environment.• Operated electrical/propane powered forklifts to assist with preventative maintenance on various equipment and to mix media components prior to upstream fermentation processing.• Worked with all team members to develop Standard Operating Procedures (SOP’s) on all current practices and equipment to assist any new coming trainees with routine operations.• Created triptic soy agar (TSA) plates and triptic soy broths (TSB) for cell cultivation/growth in order to inoculate bioreactors.• Assisted manager in developing working seeds in the laboratory for various kinds of Bacillus microorganisms in order to develop possible growth parameters for the mass production of selected strains • Streaked Bacillus vials onto TSA plates using the four quadrant methodology followed by the selection and inoculation of single isolated colonies into TSB flasks for initial cell augmentation.• Homogenized spray dried powder to determine the Colony Forming Units (CFUs) by performing multiple dilutions in order to conduct viable cell counts on TSA plates.• Conducted environmental testing on various equipment to check for microbial contamination and propose possible solutions on how to neutralize the source.

Athens, Georgia, Us

• Successfully supported the manager in adapting the brewing process, which compensated for more efficient cooling through glycol chillers and fermentation tanks.• Calculated the total amount of heat loss for a beer usage of 29,500 bbl (beer barrels)• Designed an ice bank energy storage system, which minimized manufacturing and development costs.• Determined if 60-ton Pro Chiller was beneficial or detrimental as a manner for chilling yeast fermenters.• Completed full economic analysis comparing current process expenditures to that of the proposed changes, including reactor sizing/costing, energy consumption, and reaction efficiency.• Properly constructed an Engineering Ethics Decision Matrix to account for guidelines created by ABET (Accreditation Board for Engineering and Technology)• Played an integral role in implementing ideas to advance the modification of brewing components in collaboration with engineering team members.

Athens, Ga, Us

• Utilized PCR and a binding buffer to augment Formate Dehydrogenase (fdh) gene.• Conducted restriction digestion by using BAMH1 and Sal1 to cut the enzyme at the restriction sites• Diligently transformed the PET duet 1 (Plasmid Vector) into Escherichia coli followed by purification and conversion of Escherichia coli into a protein expression.• Contributed bioconversion and biosynthesis expertise to calculate the KCAT concentration of fdh.

Bachelor'S Degree, Biochemical Engineering

Pre-Engineering