Jackie Zhao Email and Phone Number

• Supporting the Immuno-Oncology pipeline by custom tailoring antibodies for desired effector functions, pharmacokinetics or Antibody drug conjugates• Next-Gen Sequencing and analysis for antibody sequences from hybridomas and B cells• Generation of gene edited cell lines for mouse tumor models and Determined antibody sequences• Stable and transient antibody expression in CHO cells

Developing novel oncolytic newcastle disease virus as oncolytic virus for virotherapy

Developing and optimizing multiplex qPCR to detect five panel (PIK3CA, EGFR, KRAS, BRAF/NRAS) biomarker

• Established and managed an R&D lab, responsible for performing routine maintenance and calibration of equipment, stocking reagents and supplies, and tracking expenditures• Prepared next generation sequencing (NGS) libraries for whole genome sequencing and ampliseq from genomic DNA, whole genome amplified DNA, cell-free DNA in plasma, and tissue samples for the Ion Torrent Proton.• Operated the Ion Torrent Proton Sequencer, including emulsion PCR, chip preparation and loading, and initialization

• Established a Molecular Biology Core Laboratory for isolation and analysis of viral DNA and RNA that operated with 5 employees• Managed the Molecular Biology Core Laboratory, responsible for performing equipment maintenance and calibration, training users on equipment, and scheduling use of machines• Managed an in-house nucleotide and protein sequence database for Flu viruses, RSV, HSV and CMV, responsible for inputting user generated data, analyzing data, and accessing data for users• Worked in GMP environment, and took GMP training• Wrote SOPs for PCR and Sequencing• Supervised temporary employees with DNA sequencing operation• Operated Roche 454 DNA sequencer, PyroMark Q24, ABI 7900 Real Time PCR Machine, ABI 3730 DNA Analyzer, Affymetrix GeneChip Microarry Instrument, Qiagen 9604 and Beckman Coulter Biomek FX BioRobots/liquid handlers• Performed many virology techniques, including virus production, purification, titration, transgene expression, and isolation and synthesis of RNA• Performed common molecular biology techniques including, qRT-PCR, PCR, multiplex RT-PCR/F-SSCP (Single-strand conformation polymorphism) genotyping, and molecular cloning• Operated Roche 454 DNA sequencer, PyroMark Q24, ABI 7900 Real Time PCR Machine, ABI 3730 DNA Analyzer, Affymetrix GeneChip Microarry Instrument, Qiagen 9604 and Beckman Coulter Biomek FX BioRobots/liquid handlers