Zhenzhen Mo, M.S. Email & Phone Number

Alameda County, California, United States

Alameda County, California, United States

Immuno-oncology, Biotherapeutics Discovery

Redwood City, Ca

Immuno-oncology Group: Supported T-cell/dendritic cell target discovery and bispecific antibody development• Assessed T-cell and dendritic cell activation markers on PBMC by multi-color flow cytometry• Immunophenotyped dendritic cells, splenocytes, dissociated tumor cells, Kupffer cells and platelets • Used FACS Melody to sort cells having varying target expression levels• Evaluated cellular internalization of antibodies using Incucyte • Assessed immunogenicity of antibodies… Show more Immuno-oncology Group: Supported T-cell/dendritic cell target discovery and bispecific antibody development• Assessed T-cell and dendritic cell activation markers on PBMC by multi-color flow cytometry• Immunophenotyped dendritic cells, splenocytes, dissociated tumor cells, Kupffer cells and platelets • Used FACS Melody to sort cells having varying target expression levels• Evaluated cellular internalization of antibodies using Incucyte • Assessed immunogenicity of antibodies by CDC and ADCC: C1q binding by ELISA and flow cytometry, CDC with rabbit complement, and ADCC using reporter cell assay• Validated antibodies on targets expressed on primary and cultured cells• Evaluated activity of ligands and bispecifics using reporter cell lines and activation of PBMCs and DCs• Developed mixed-culture assays to evaluate the preferential binding of modified antibodies to Fcy receptors• Isolated PBMC and iDC from leukopak• Quantified cytokines using Meso Scale Discovery's multiplexed platform• Completed demo for Cytek Aurora, Nanostring nCounter and Nanostring GeoMx Digital Spatial Profiling• Presented research results in internal and external meetingsMolecular Biology & Protein Group: supported research projects by cloning and protein production• Performed molecular cloning procedures: transform Mach1 competent cells, miniprep, maxiprep• Supported protein production: transfect HEK293F using ExpiFactamine, assess protein expression using ForteBio Octet, purify proteins by column chromatography, quantified protein using Thermo Nanodrop• Carried out QC assessment of proteins using Agilent Bioanalyzer and Tycho Nanotemper (received training for Agilent 1260 Infinity II HPLC)• Optimized RNA extraction for low number of primary cells• Extracted RNA from mouse spleen for gene expression analysis using Nanostring nCounter• Determined Fcy receptors polymorphisms through nested PCR and sequencing• Trained new staff on assay protocols and instruments Show less

Redwood City, California, United States

• Full-time caregiver of my newborn baby in Taiwan• Published an original research journal article as co-first author (Journal of Nutrition on Feb 10, 2020)

Davis

Human clinical study: It is a double-blinded, placebo-controlled, dietary intervention study aiming at examining whether a high-fat meal causes monocyte activation and whether concomitant intake of DHA or blueberries suppresses the postprandial inflammation.• Informed the subjects the dietary restrictions of the study • Explained the written consent forms to subjects in one-to-one meetings• Advertised for the study on the UC Davis campus• Carried out whole blood assay to… Show more Human clinical study: It is a double-blinded, placebo-controlled, dietary intervention study aiming at examining whether a high-fat meal causes monocyte activation and whether concomitant intake of DHA or blueberries suppresses the postprandial inflammation.• Informed the subjects the dietary restrictions of the study • Explained the written consent forms to subjects in one-to-one meetings• Advertised for the study on the UC Davis campus• Carried out whole blood assay to evaluate cytokine productions in blood in-vitro• Performed cytokine quantification on the Meso Scale Discovery (MSD) platform Project 1: Direct and indirect quantification of endotoxin in human plasma• Researched and decided on the instrument, reagents, and labware best suited for the quantification of endotoxin in human plasma • Developed sample treatment methods to remove interfering components in plasma• Developed controls to verify the appropriateness and effectiveness of sample treatments • Assessed endotoxin presence using endotoxin neutralization capacity assayProject 2: Assay developments to use Cellufine ™ • Optimized conditions to evaluate the use of Cellufine ™ beads (cellulose resin cross-linked to polylysine) in removing endotoxin from human plasma• Developed methods to quantify endotoxin bound to the resin Show less

Cell Biology and Assay Development Department: Generated primary data crucial for grant applications. Projects include plant-made vaccines, antibodies, nanobodies, and immunoadhesins.• Performed antibody characterizations, including specificity (ELISA), functionality (FRET and cell based potency assay), size distribution (Western and HPLC), and glycosylation analysis• Developed assays and tested serum samples from cynomolgus macaque challenged with Bacillus anthracis spores on Meso… Show more Cell Biology and Assay Development Department: Generated primary data crucial for grant applications. Projects include plant-made vaccines, antibodies, nanobodies, and immunoadhesins.• Performed antibody characterizations, including specificity (ELISA), functionality (FRET and cell based potency assay), size distribution (Western and HPLC), and glycosylation analysis• Developed assays and tested serum samples from cynomolgus macaque challenged with Bacillus anthracis spores on Meso Scale Discovery ECL platform • Performed cell-based assays, including toxin neutralization assay (TNA), antibody-dependent cell-mediated cytotoxicity (ADCC), cytopathic effect (CPE) of viral invasion, induction of multipotent cell differentiation, and cell penetrations• Contributed to the company’s human pre-clinical trial preparations• Trained new employees on assay protocols and instrumentsMolecular Biology Department: • Completed an independent project on the creation of an immunogenic molecule with multiple Fc fragments • Carried out molecular cloning to generate various new constructs• Purified DNA from plants• Purified RNA and generated cDNA by reverse transcriptionProcess Development Department: • Established HPLC procedures and carried out HPLC size exclusion chromatography to fractionize and analyze PBI220 (an immunoadhesin against Bacillus anthracis toxin) • Presented the findings to upper management and external consultants• Developed and completed experiments to evaluate product stability • Optimized culturing procedure and established the growth curve of mouse macrophage (RAW 264.7) cells for a product release assayTissue Culture Department: • Generated transgenic whole plants from transfected leaf disks • Screened leaf tissue to isolate plants transgenic for PBI-220 Show less

Hayward, California, United States

• Completed high quality FACS (fluorescence-activated cell sorting) staining for flow cytometric analysis of gamma-irradiated mammalian cells• Systemized database to access cell lines more efficiently

• Conducted literature searches related to cell aging• Originated research in quiescence/senescence in planarian cell aging• Carried out PCR, mRNA isolation, cDNA library preparation to study gene expression

• Optimized antibody pairs crucial to supervisor’s research in acute myeloid leukemia • Independently operated BD LSRII benchtop flow cytometer • Proficient in FACSDiva and Flowjo

Bachelor Of Arts - Ba, Molecular And Cell Biology With Emphasis In Biochemistry

Activities and Societies: China Dance Theatre International Society of Business and Foreign Affairs

Master Of Science - Ms, Nutritional Biology

Activities and Societies: Honor Society