Jakub Zmajkovic Email & Phone Number

Vienna, Austria

Genetic and pharmacological dependencies in STAG2-mutated cancersI leverage shRNA- and CRISPR-based genetic screens to systematically discover and functionally validate novel therapeutic targets in the essential gene space in STAG2-mutated cancers.• Established a protocol for transient delivery of dual-labeled sgRNA guides for efficient gene editing.• Engineered human cell lines utilized for genetic screening assays.• Designed next-generation shRNAmir library for RNAi… Show more Genetic and pharmacological dependencies in STAG2-mutated cancersI leverage shRNA- and CRISPR-based genetic screens to systematically discover and functionally validate novel therapeutic targets in the essential gene space in STAG2-mutated cancers.• Established a protocol for transient delivery of dual-labeled sgRNA guides for efficient gene editing.• Engineered human cell lines utilized for genetic screening assays.• Designed next-generation shRNAmir library for RNAi genetic screens (collaboration with Stefan Ameres lab, MPL Vienna).• Participated in the development and characterization of ARTi - an ultrapotent, synthetic, and off-target-free RNAi system, reported in eLife (collaboration with Boehringer Ingelheim RCV).• Supervised two graduate research students: managed day-to-day activities, planned and analyzed experiments, monitored project progression and milestone achievements, coordinated with the PI. Show less

Basel Und Umgebung, Schweiz

Molecular origin of myeloproliferative syndromesI used the combination of human genetics and molecular biology to fully elucidate the molecular mechanism of a novel gain-of-function mutation in the EPO gene in patients with familial erythrocytosis.• Established CRISPR-Cas9-gene editing protocol and employed it to engineer a cellular model of a disease.• Developed qPCR-based assay for assesing gene editing efficiency.• Generated cell lines used for the development of… Show more Molecular origin of myeloproliferative syndromesI used the combination of human genetics and molecular biology to fully elucidate the molecular mechanism of a novel gain-of-function mutation in the EPO gene in patients with familial erythrocytosis.• Established CRISPR-Cas9-gene editing protocol and employed it to engineer a cellular model of a disease.• Developed qPCR-based assay for assesing gene editing efficiency.• Generated cell lines used for the development of selective JAK2 inhibitors (manuscript in revision).• Performed transplantation assays using primary murine hematopoietic and progenitor cells.• Wrote, edited, and finalized a first-author manuscript published in the New England Journal of Medicine; co-authored 3 other publications in top-tier journals. Show less

Vienna

Development of therapeutic monoclonal antibodies• Member of a cross-functional mAb production team that supported other pre-clinical teams.• Responsible for the design and execution of cloning strategies to express fully humanized IgG antibodies under GLP conditions and following SOPs.

Vienna, Austria

Mechanisms of cell polarity and asymmetric cell division • Examination of the role of proteins involved in cell polarity using C.elegans one-cell embryos using confocal spinning disc microscopy and wide-field light microscopy.

Basel Und Umgebung, Schweiz

Molecular mechanisms of breast cancer cell migration (Nancy Hynes)• Study of the mechanisms underlying breast cancer cell migration (master thesis).• Established high-throughput imaging of transwell migration assays (with Markus Wartmann, NIBR Basel).

Doctor Of Philosophy - Phd, Biomedical Research, Magna Cum Laude

Molecular origin of myeloproliferative disorders (Radek Skoda) Doctoral dissertation: Genetic studies of hereditary myeloproliferative.

Master Of Science (M.Sc.), Molecular Biology, With Honors

Master thesis: Memo-an ErbB2 effector plays a role in S1P-induced signaling, migration and in embryonic development Mentor: Nancy E..

Exchange Student, Molecular Biology

Bachelor Of Science (B.Sc.), Biology, General

Bachelor thesis: Quantitative Reverse-Transcription PCR: Application for detecting micrometastasis Mentor: Lucia Kučerová, Laboratory.