강찬영 Email & Phone Number

Education2017.03-2023.02 Integrated M.Sc. &Ph. D. in ChemistryHanyang University, Seoul, South KoreaAdvisor : Dr. Sangsu Bae2013.02-2017.02 B.Sc in ChemistryHanyang University, Seoul, South KoreaGrant2018.03 – 2023.02 Global Ph.D Fellow, National Research Foundation of Korea (NRF)Skills and Techniques Genome editingCRISPR-based genome editing of in vitro or mammalian cellsKnock-out or knock-in of a specific gene using CRISPR-Cas9Specific gene knockout or inducing point mutation using base editors and prime editorsSequencingIllumina sequencing – MiniSeq instrument operation, Library preparation Nanopore Sequencing – Minion instrument operation, Sample preparation (PCR sample, Cas9 enriched Sample, RNA sample), Base calling, Align to reference genomeCloningCloning using restriction enzyme / Gibson assembly / Cas9-based methodRNA worksTrizol, Column or beads-based RNA extraction, cDNA synthesize, qRT-PCRIn vitro transcription of mRNA or gRNAHave experience using Cas13 for knockdown or RNA editing in mammalian cellsProtein worksWestern blotProtein purification with his-tagMitochondrial worksMitochondria extractionWhole mitochondria sequencing using Illumina sequencing or Nanopore sequencingCell culture and TransfectionCancer cell line or immortalized mammalian cell cultureLipid or Cation chemical based transfection – Lipofectamine, PEI, JetOptimus, Turbofect, etcElectroporation – Neon(Thermo Scientifc), Amaxa(Lonza), Gene pulser(Bio-rad) Poster presentation 2022.05 KSBMB international conference- In vivo genome editing in single mammalian brain neurons through CRISPR-Cas9 and cytosine base editors

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  Postdoctoral Reseasrcher/박사후 연구원

  고려대학교

  2023 - Present