PRINCE: Proteomics-based Research Initiative for Non-Cytochrome P450 Enzymes

Proteomics-based Research Initiative for Non-Cytochrome P450 Enzymes (PRINCE) is a research collaboration between Washington State University and multiple pharmaceutical companies. The overarching objective of PRINCE is to enable prediction of drug metabolism mediated by non-cytochrome P450 (non-CYP) enzymes. The research utilizes quantitative mass spectrometry proteomics for: 1) The characterization of differential tissue expression, interindividual variability, and inter-species differences in non-CYP enzymes 2) In vitro to in vivo extrapolation (IVIVE) of non-CYP metabolism for predicting drug clearance and fractional contribution of individual enzymes 3) Physiologically-based pharmacokinetic (PBPK) modeling of drugs metabolized by non-CYP enzymes