X Gen

X Gen developed the ‘ted-Technology’ for NGS library construction from limited and degraded clinical samples. Similar to a qPCR assay, our methods are extremely sensitive and very easy to automate. Key applications are cancer screening, liquid biopsy, single cell analysis, and meta genomic pathogen sequencing. All methods are ‘single tube’ and completed in 2 hours. Cancer Screening with combined DNA&RNA Panels Precious biopsies provide very limited samples for biomarker identification. X Gen’s combined DNA&RNA library preparation kit (ted-CapALL) enables the use of combined DNA&RNA target enrichment panels. With a unified DNA&RNA analysis workflow, a single assay identifies DNA and RNA mutations to support treatment decisions. Analysis of cfDNA and cfRNA in Liquid Biopsy Samples Initial liquid biopsy assays focused on analyzing circulating free DNA (cfDNA). More assays now focus on including circulating free RNA (cfRNA) for early cancer detection. X Gen’s ‘ted-CapALL’ kit successfully creates libraries from difficult liquid biopsy samples like enriched exosomes or serum. Whole Genome amplification The ‘ted-technology’ enables single cell whole genome amplification (WGA) from single cells. The ‘ted-WGA’ uniformity is superior compared to the most frequently used technologies, thus providing higher resolution for mutation detection. An ideal application is high-resolution copy number analysis to characterize circulating tumor cells. Metagenomic DNA&RNA Pathogen Sequencing Detection of causative pathogens so far required DNA- and RNA-specific pathogens. X Gen’s combined DNA&RNA library construction methods detect DNA and RNA pathogens in clinical samples like lavage fluids (BALF) and throat swabs. Metagenomic pathogen sequencing allows the detection of causative pathogens as well as the characterization of the host response to the infection and treatment.