Akbor Hossain Email & Phone Number

Newtown, New South Wales, Australia

Sydney, New South Wales, Australia

The project involved in understanding the role of MicroRNAs in cutaneous squamous cell carcinoma (cSCC) and its precursor lesions and identify possible miRNA biomarkers.o Perform a global miRNA microarray analysis in cSCC patient sampleso Extraction of DNA/RNA o Validation of miRNA identified from microarray using RT-qPCRo Transfection of miRNAs in cSCC cell lines for functional characterisation o Perform proliferation assay, 2D colony formation assay, wound healing assay and invasion assayo Prediction of miRNA binding targets and subsequent validation using RT-PCR and Western blots

At Associate Professor Ulrike Kappler's lab I participated in two research projects. One of the project involved in understanding the survival mechanism of Haemophilus influenzae in response to reactive oxygen species (ROS). While the other project aimed at determining the role of thiosulfate for the survival and energy metabolism on Ruegeria pomeroyi.o Preparation of growth media for bacterial cultureo Growing and maintaining bacterial culture at large-scale o Cloning and Expression of three enzyme (MsrAB, Trx and Trx-R from H. influenzae) in Escherichia Coli.o Purifying the enzyme using fast protein liquid chromatography (FPLC)o Perform quality control of extracted protein lysate o Characterising the function using enzyme kinetic assayso Growing R. pomeroyi in different carbon and thiosulfate compoundo Extraction of whole protein lysate and SDS-PAGE separation o Gel digestion and Zip-Tip Protein clean upo Run LC-MS/MS and perform QC of MS data and submission to Mascot

Bachelor Of Science (Honours), Biochemistry And Molecular Biology, Class 1

Bachelor Of Science - Bs, Biochemistry And Molecular Biology