Aaron Ruby Email & Phone Number

Miami, Florida, United States

• Revitalized and oversaw high-throughput robotic liquid handling processes for NGS library prep from RNA.• Trained team members on high-throughput protocols, overseeing preparation of 50-100 libraries monthly.• Generate, prepare, and load libraries for sequencing, contributing to high-quality research data.• Responsible for developing automated RNA extraction from Wastewater for COVID tracking and transformation into libraries for NGS sequencing• Led training and SOP development for sample quality control, initiating comprehensive QC procedure overhaul, and managed QC procedures for external and in-house samples, including RNA, DNA, and NGS Libraries.

Miami, Florida, United States

Responsible for mastery of all Beckman lab instrumentation, accurately documenting all GLP lab activity, performing experiments according to written protocols, collecting and analyzing data, writing reports for internal and external use.• Led and designed studies for the feasibility of new reagents for use.• Co-led multi-day value assignment testing, including generating, collecting, analyzing and formatting data for customers.• Led and completed a system verification test report by combining data from multiple documents and summarizing findings.• Led team for lab reorganization and reconfiguration.

North Besthesda, Maryland

I was an accomplished fencing coach with regional and national medalist students. I led practices for youth and adult fencers, gave individual lessons to students, and develop and implement drills and exercises of my own. I worked to improve the physical and mental skills of my students, I coached fencers at local, national, and international competitions, and help run the club.

Rockville, Maryland, United States

Optimized a high-throughput robotic instrument design and performed experiments to test individual portions of the high-throughput workflow, document and presented findings to supervisors and peers, used the workflow for the testing of COVID spike protein RNA.• Performed manual experiments for bacterial cloning, DNA linearization and RNA generation in order to optimize for use on a Tecan robotic instrument able to create 192 constructs in a single run via cloning, transformation, bacterial plating, colony picking, DNA purification, RNA production via IVT• Transfected RNA into live cells for downstream applications, such as RNA potency assays. • Utilized robotic workflow for library generation, AI generated sequences, and COVID mutant research

Rockville, Md

• Developed and troubleshot multiple assays for PCR, designed and spearheaded experiments for assay development, including the design of primers and probes for qPCR assays.• Vital member of the assay team developing a in-vitro diagnostic device for bacterial detection.• Documented work, wrote technical reports, and presented findings to supervisors and peers.•Extracted DNA from FFPE tissues for use in assay development of a novel SNP amplification method.Publications“T-blocker: A simple and robust probe-free qPCR assay to detect somatic mutations down to 0.1% frequency” Hanyoup Kim, Aaron E. Ruby, Harini G. Shandilya, Arvind K. Virmani, Nandita Rahman, Christina M. Strange, Jarkko Huuskonen

Gaithersburg, Maryland, United States

• Performed robotic and manual PCR testing on hundreds of DNA samples per week• Responsible for the preparation and testing of patient samples in order to identify genetic diseases, and diagnose them in our patients.

Gaithersburg, Maryland

• Optimized a robotic in-gel digester for tryptic digestion of Coomassie stained protein gel bands using a single known protein or mix of several known proteins.• Separated proteins using SDS-PAGE gel electrophoresis• Tested the effectiveness of protein digestion using a robotic in-gel digester with multiple experiments. • Ran a mass spectrometer to evaluate protein digestion based on yield. • Compared current digestion methods with the in-gel digester using proteins spiked into mAb, simulating Host Cell Proteins

National Institutes Of Health

I have completed a nine month workshop at the National Institutes of Health (NIH) that bridges the gap between the level of laboratory skills biology graduates have acquired, to the proficiency level needed to be productive in a research laboratory setting. The course consists of fifteen hands-on laboratory sessions (seventy-five contact hours) covering the latest relevant laboratory techniques in molecular biology. These selected methods have been identified by NIH scientists and private industry representatives to be techniques that research technicians would implement on a day-to-day basis. It also includes weekly lectures on the pertinent topics of study.

I worked in the lab of Dr. Patrick N. Breysse with Christine Torrey taking and categorizing second-hand smoke samples for study.

Haverford, Pa

I worked in the Lab of Dr. Iruka Okeke in the Biology Department of Haverford College and conducted experiments on the autoaggregation of Escherichia coli pertaining to the Hra1 protein. This position included maintenance of a Human Epithelial cell culture, and the staining and photographing of multiple E. Coli clones. This data was then presented as a poster.

Master Of Science - Ms, Biostatistics

Activities and Societies: Member of Beta Sigma Chapter of Delta Omega, the Public Health Honor Society

Bachelors Of Science: Chemistry, Chemistry

Activities and Societies: Varsity Fencing Team, Haverford College Humtones, Peer Awareness Facilitator Committee Member, Peer Awareness.