Aarthi Rathnasabapathi Email & Phone Number

Chennai, Tn, In

Chennai, Tamil Nadu, India

Migration and Maintenance of QC data in LIMS systems for Pharma and Consumer clients

Chennai Area, India

Analysis of charge variantsSECProtein Quantification by UV280 MethodPeptide mappingGlycan AnalysisRP-HPLC was performed for standards and examined with different protocolsDeveloped a new method for Pro-A affinity chromatography for a bio-similar* and drafted the reportPro-A affinity chromatography was performed for different in-house samples

Bengaluru Area, India

Tested compounds on Aβ-42 levels in neuroblastoma cells by ELISA and cytotoxicity testing by ATP assayScreening of compounds against inflammation specific targets by non-cell based fluorescence assaysAnti-diabetic drug screening in C2C12 mouse myotubes, liver and skeletal muscle samples by western blottingAnti-diabetic drug screening in rat hepatocytes primary culture, HEK293 and HCT116 cell lines by ELISACytotoxicity testing of drugs (MTT) in multiple cell linesOptimization of eosinophil shape change assay for respiratory diseases by FACSScreening compounds against lung diseases by Malachite green assayOptimization of enzyme kinetic assay against Tuberculosis: Testing enzyme activity, Km, Vmax analysis, analysis of substrate and cofactor stability, screening of compounds, IC50 studies and data analysisOptimization of GCSF activity assay in m-nfs60 cellsAssayed transaminase mediated chiral amine synthesis by HPLCCloning Projects:Production of recombinant protein by Baculovirus Expression system – Cloning and transformation, transfection of the recombinant bacmid DNA into insect cells, followed by tittering of the baculovirus stock to express the recombinant protein.Design and cloning of the gene of interest by Viral based Expression system – Designing of retroviral based pci-neo vector, transformation of the vector into DH5α cells, restriction digestion, ligation followed by transformation and colony screening by PCR, transfection in mammalian cell line.Design and synthesize gene encoding the enzyme from two different bacterial cultures for the production of chiral amines by whole cell biotransformation methodOther Skills:An active member of SOP core teamLaboratory manager in maintenance and calibration of analytical instruments

Edinburgh

Quantification of DNA and RNA using Screen Tape technology and Benchmarking against Agilent’s Bio-Analyzer LabChip technology

Edinburgh, United Kingdom

Designing macromolecular pro-drugs of colchicine, colchicine-amide, anthraquinone. Indomethacin, dexamethasone and its derivatives and testing its toxicity in mammalian cell lines

Master Of Science, Drug Design & Biomedical Science

B.Tech, Industrial Biotechnology