• Expressed recombinant methyltransferase proteins (Bacterial transformation and culture)• Purified recombinant proteins using Fast Liquid Chromatography (FPLC) (affinity, ion exchange, size exclusion)• Determined optimal storage buffer components for protein using a Differential Scanning Fluorimetry assay (DSF)• Employed various protein crystallization techniques (setting up trays, collecting crystals and seeding)• Primer design and PCR for mutagenesis• Extraction and purification of plasmid DNA with Minipreps• Assessed small-molecule mediated inhibition using high-throughput assays• Compared enzyme activity of wild-type with mutant protein using a scintillation assay (radiometric, proximity-based)• Liquid Chromatography-Mass Spectrometry (LCMS)• Gel electrophoresis• Gravity columns, batch binding

Post-baccalaureate Research Opportunity to Promote Equity in Learning

• Employed multiple molecular biology techniques to validate demethylase constructs used for a bioluminescence cell culture assay • Mammalian cell culture maintenance • Transfection of DNA constructs • Carried out varying lysis protocols for the isolation of nuclear and cytosolic fractions • Western blot • Extraction and purification of plasmid DNA • Bacterial transformation • PCR primer design

• Interpreted electrocardiograms with accuracy • Escalated abnormal rhythms following nomenclature and criteria• Notified supervisors of lethal arrhythmias with a sense of urgency

• Received, input into the system, filled and sold prescriptions• Managed inventory by receiving and organizing incoming orders• Maintained positive customer service while developing rapport with regular clients

• Received, input into the system, and sold prescriptions• Managed inventory by receiving and organizing incoming orders• Maintained positive customer service while developing rapport with regular clients

• Assisted in organizing the floor setup and item management• Worked towards speedy and personalized customer service

Preliminary characterization of sorbents for the purpose of remediation of inorganic contaminants in water. Method consisted on the use of MP-AES and modeling in Excel Solver.

• Set up reagents and materials needed for labs (Mostly for organic chemistry but also for quantitative chemistry classes)• Prepared solutions and properly tagged and disposed of waste following protocols• Cleaned and organized stockroom and labs –including all hoods• Kept inventory and tracked incoming packages and gas tanks• Worked both independently and in coordination with other assistants• Trained new employees to conduct the same procedures