Adilya Rafikova Email & Phone Number

Toronto, Ontario, Canada

- Conduct biochemical assays: ELISA, NADH, Western Blot- Collect and process animal tissue: Protein lysate preparation, microtome and cryostat dissection on rodent brains- Rodent handling, dosing (OP, IN, IP), bleeding- Perform stereotactic surgery on rodents and post-operative care- Conduct behavioral testing according to protocols and timelines-Use of stereo investigator for cell counting in the substantia nigra (SN)

Toronto, Canada Area

• Handling various human samples• DNA extractions from blood, saliva, tissues, FFPE• RNA extraction from blood, tissues, FFPE• Sample entering and documenting through LIMS system

Toronto, Canada Area

Toronto, Canada Area

• Validated SNP and Indel mutations by PCR and Sanger Sequencing• Designed and validated primers for hot spot mutations in colorectal cancer• Shipped and received human DNA samples • Kept neat records for all performed experiments • Maintained the lab and performed inventory, and placed orders • Extracted RNA and DNA from colorectal cancer cell lines for further experiments• Quantified gene expression in cell lines and human samples using probe based and SYBR protocols• Tested multiple Live cell imaging systems for cell proliferation and scratch assay• Tested Droplet digital PCR system with cell line DNA and human DNA

Toronto, Canada Area

• Performed and analyzed immunological assays: HA, HAI, ELISA• Extracted RNA and performed gene expression analysis from tissues, urine, blood and feces• Performed Virus propagation, characterization and titration in chicken eggs• Maintained and performed various assays on Vero and MDCK cell lines - plaque assay and TCID50 • Handled animals (ferrets and mice) – infection, blood collection, terminal bleed/tissue collection and processing• Processed animal blood – sera and PBMC extraction

Whitby

Media preparation, Growth Promotion, Autoclave validation, Identification of microorganisms with the use of API strip, Environmental Monitoring, Cleaning Validation

Toronto, Canada Area

• Critical review of scientific literature regarding lung cancer• Data mining and curation• Data archiving and data management for import into a database• Generated periodic progress reports• Attended and reported at scientific meetings• Assisted in maintaining lung cancer cell lines

Toronto, Canada Area

• Lung Data Portal project• Drafted a project abstract for a conference and sections for a scientific grant proposal• Collaborated with research scientists, clinicians and software developers/database administrators• Critical review of scientific literature regarding lung cancer• Data mining and curation

Performed Microbial Limits Tests to detect yeast and molds, total plate count (TPC) and pathogens such as: E. coli, S. aureus, Salmonella and P. aureus based on USP, EP and client's Methods Conducted Environmental Tests for water to detect coliforms, Pseudomonia species, yeast and molds, total plate count (TPC), and for air to detect yeast and molds and total plate countCollected and analyzed data, test result/readings and interpreted and compared them to standards (USP, EP, and In-House and Client's methods)Identified different types of micro-organisms using API and Gram-stainingPerformed sampling, dilutions, percent composition analysisPrepared rich and selective media, buffers and solutionsPerformed Growth Promotion on mediaPerformed stock culture count of bacteria and yeast and molds on biweekly basisMaintained a detailed record of all test results according to GMP guidelinesFamiliar with Antibiotic Assay and Sterility test; Preparation and sterilization of rich and selective media

Performance testing of prepared media and reagents using standardized bacteriaBacterial identification through biochemical testing, e.g. Enterotube, API 20Using aseptic techniques in bacterial stock culture preparationMonitoring microbiology laboratoryTrained to use light microscope and autoclaveBacterial slide preparation and differential staining(Gram, Endospore)

Master'S Degree, Molecular Biology

Honours, Biology, 7.9 Out Of 9.0

First Class

Advanced Diploma, Biotechnology, 4.0

Activities and Societies: SMILE MentorHigh Honours

Education record