Alaa Salih Email & Phone Number

* Experience with multiple cell culture models and development of engineered cell lines, such as (HEK cells, hBMDM, WT BMDM, TREM2, hPMC,PBMC,iPSC ,and Microglia)* Design and perform complex liquid handling workflows using automated liquid handling platforms, such as Bravo and IDOT for accurate, efficient, and cross contamination- free compound transfer* Perform biochemical, immunoassays and cell-based assays using imaging, real-time PCR detection systems, and plate readers* Screen chemically diverse compounds, computationally selected molecules, and FDA approved bioactive compounds libraries for potential therapeutic development* Experience with protein detection and molecular biology techniques (Western blot, imaging, flow cytometry, RT-PCR,ddPCR, qPCR, MiSeq, ligand binding assays, Octet, pSyk ELISA, MSD, and RP- HPLC) * Developed different assays using different molecular biology techniques (in Vivo&in Vitro) * Data analysis and visualization using different software, and hardware• Experience with multiple cell

Beverly, Massachusetts, United States

• Viral RNA and DNA extraction (Covid-19 and BRCA) using saliva and nose swab and MiSeq• Molecular Biology (RNA and DNA sequencing, Nucleic Acid Extraction from Fresh Tissue, FFPE, Blood, Cell Culture, Transfection, Co-Immuno precipitation, Western Blot, and Protein modification,) • Optimized 3’RNA sequencing with Illumina platform, NGS, and Sanger• Optimizing library preparation and nucleic acid extraction from blood FFPE, Fresh Frozen Tissue)• SOP generation and implementation • Managed and trained incoming interns• CLIA Validation FDA,EU for BRCA and Covid-19• Managed general lab regulations and instrument maintenance

Beverly, Massachusetts, United States

• Grew and maintained stock cultures of aero-tolerant and facultative anaerobic bacterial strains (laboratory and clinical).• Performed functional enzymatic tests on target bacteria to find the best treatment for acne• Inoculated and grew protein production cultures of strains containing recombinant plasmids• Extracted and purified proteins and functional enzymes of interest expressed in transformed host bacteria strains• Separated and visual purified proteins on Native and SDS polyacrylamide gels• Optimized production of proteins from recombinant plasmid constructs in broth media.