Alexzander Sharp Email and Phone Number
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Highly motivated molecular biologist with expertise uncovering transcriptional mechanisms. Strengths include NGS as well as R, Python, and the Unix/bash shell. Adept at experiments and techniques involving mammalian cell culture, as well as in biochemical techniques such as protein purification and in vitro binding assays.
Mircore 501(C)(3)
View- Website:
- mircore.org
- Employees:
- 15
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EducatorMircore 501(C)(3) Aug 2018 - PresentAnn Arbor, Michigan, United States-Taught students cancer and disease-related molecular biology as well as bioinformatics techniques such as R and Bash, including the alignment and visualization of raw sequencing data.-Taught students how to use online databases and resources such as GEO/GEO2R and StringDB-Evaluated genes for potential use as biomarkers or for pharmaceutical targeting with students in program -Utilized team-building techniques to foster a cohesive learning environment and enhance student success. -
Graduate ResearcherUc San Diego Sep 2016 - Mar 2023San Diego, California, United States-Conducted research on the regulation of transcription in the context of estrogen-dependent transcription in MCF7 breast cancer cell lines in the Rosenfeld Laboratory, Department of Cellular and Molecular Medicine-Cultured mammalian cells, performed cell-based assays and experiments, and prepared sequencing libraries using DNA and RNA workflows, including ChIP-seq, PRO-seq, and CUT&TAG/ATAC-seq with NEBNext product suites-Constructed plasmids through molecular cloning using restriction and Gibson assembly methods for applications such as lentiviral transduction in trangenic expression, the suppression of protein production using shRNAs, and the creation of mutations via CRISPR/Cas9-Performed statistical analyses and bioinformatic data processing using R, Python, the Unix/Linux environment and GraphPad Prism.-Purified protein using His and GST affinity chromatography for biochemical assays-Used immunohistochemistry and in vivo fluorescent tags to assess protein expression and localization.-Collaborated with other laboratories on projects, performing RNA FISH and CUT&TAG on lymphocytes and monocytes, respectively, and prepared ChIP-seq and ATAC-seq libraries from neurons differentiated from IPSCs.-Used flow cytometry to isolate lymphocytes from murine spleen tissues and to profile cells for markers of cellular differentiation.-Led discussion sections for courses including Bioinformatics Laboratory, Cellular Mechanisms of Human Disease, and Human Anatomy and Physiology.-Supervised and mentored Research Assistants to perform experiments and laboratory maintenance tasks-Assisted in maintaining laboratory safety standards and laboratory equipment -
Research AssistantUniversity Of California, Davis Jan 2015 - May 2016Davis, California, United States-Performed research on DNA repair mechanisms, focusing on protein expression and purification.-Used HAP chromatography, size exclusion, and anion exchange with FPLC to purify RPA complexes from E. coli.-Employed two-step affinity purification techniques to purify BRCA2 from SF9 and S. cerevisiae (yeast) using MBP and His tags.
Alexzander Sharp Skills
Alexzander Sharp Education Details
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Biological Sciences
Frequently Asked Questions about Alexzander Sharp
What company does Alexzander Sharp work for?
Alexzander Sharp works for Mircore 501(C)(3)
What is Alexzander Sharp's role at the current company?
Alexzander Sharp's current role is Research Molecular Biologist (PhD).
What is Alexzander Sharp's email address?
Alexzander Sharp's email address is as****@****vis.edu
What is Alexzander Sharp's direct phone number?
Alexzander Sharp's direct phone number is +153075*****
What schools did Alexzander Sharp attend?
Alexzander Sharp attended University Of California, San Diego, Uc Davis, Santa Rosa Junior College.
What skills is Alexzander Sharp known for?
Alexzander Sharp has skills like Microsoft Word, Western Blotting, Microsoft Excel, Public Speaking, Microsoft Office, Teaching, Cell Culture, Molecular Biology, Pcr, Protein Purification, Fplc, Agarose Gel Electrophoresis.
Who are Alexzander Sharp's colleagues?
Alexzander Sharp's colleagues are Alekhya Kotha, Abha Gupta, Catherine Hardwick, W R, Valarie Thomas, Aadit Mathur, Medha Mamidipaka.
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Alexzander Sharp
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