Graduate Researcher
San Diego, California, United States
-Conducted research on the regulation of transcription in the context of estrogen-dependent transcription in MCF7 breast cancer cell lines in the Rosenfeld Laboratory, Department of Cellular and Molecular Medicine-Cultured mammalian cells, performed cell-based assays and experiments, and prepared sequencing libraries using DNA and RNA workflows, including ChIP-seq, PRO-seq, and CUT&TAG/ATAC-seq with NEBNext product suites-Constructed plasmids through molecular cloning using restriction and Gibson assembly methods for applications such as lentiviral transduction in trangenic expression, the suppression of protein production using shRNAs, and the creation of mutations via CRISPR/Cas9-Performed statistical analyses and bioinformatic data processing using R, Python, the Unix/Linux environment and GraphPad Prism.-Purified protein using His and GST affinity chromatography for biochemical assays-Used immunohistochemistry and in vivo fluorescent tags to assess protein expression and localization.-Collaborated with other laboratories on projects, performing RNA FISH and CUT&TAG on lymphocytes and monocytes, respectively, and prepared ChIP-seq and ATAC-seq libraries from neurons differentiated from IPSCs.-Used flow cytometry to isolate lymphocytes from murine spleen tissues and to profile cells for markers of cellular differentiation.-Led discussion sections for courses including Bioinformatics Laboratory, Cellular Mechanisms of Human Disease, and Human Anatomy and Physiology.-Supervised and mentored Research Assistants to perform experiments and laboratory maintenance tasks-Assisted in maintaining laboratory safety standards and laboratory equipment
