• Extract and purify DNA and RNA at various scales and from different material types using both manual and automated systems• Perform QC analyses (RNA/DNA integrity analysis) to ensure that results meet specifications• Completes all paperwork completely and accurately for QA/QC and GLP purposes using Good Documentation Practices (GDP) and updates computer tracking systems, as required• Revise SOPs and makes necessary recommendations to improve efficiency and quality• Responsible for maintenance and calibration of laboratory equipment• Manage daily lab activities- review folders and paperwork, order lab supplies and reagents, create schedules to ensure all work is completed on time• Test and validates new assays and procedures

Responsible for identifying activation of oncogenes in cells infected with an HIV-1 based virus via DNA extraction, purification, sequencing and BLAT analysisMaintained mammalian cell population, produced virus, and infected cells in laminar flow hoodPerformed plasmid propagation and purification, ran agarose gels, and gel extractionsOther duties as assigned, including basic laboratory maintenance, making agar plates, preparing solutions and media, and maintaining a daily notebook.

Characterized Immunoglobulin G (IgG)-binding domains of a protein expressed by group G streptococci by engineering different generic constructs with varying numbers of IgG-binding domains and determining their IgG-binding potentials via enzyme linked immunosorbent assay (ELISA).Performed PCR colony screening, DNA cloning and bacterial transformationsResponsible for SDS-PAGE/Western blotting, nickel column purification, and spectrophotometry of DNAMaintained daily notebook and performed other duties as assigned

Determined the structure-function relationships of bacterial albumin-binding domains of group G streptococci using ELISAsPerformed SDS-PAGE/Western blotting and maintained bacterial cultures Other duties included basic laboratory maintenance and maintaining a daily notebook