• Conducted research on mutant huntingtin (mHTT) and its role in the pathology of Huntington’s disease (HD), concentrating on the identification of therapeutic targets and intervention strategies, with a focus in the neurovascular unit and an emphasis on studying endothelial cells and their interactions within the blood-brain barrier.• Maintained iPSC and differentiated cell cultures, ensuring sterile conditions and monitoring growth characteristics.• Developed, optimized, and validated experimental designs and standard operating procedures (SOPs) to improve data reliability and experimental reproducibility in multiple projects.• Assisted in a collaborative effort with a graduate student to optimize a 3D organ-on-a-chip device, achieving a functional blood-brain barrier model for use in disease modeling experiments.• Drafted detailed research summaries and reports for internal presentations, external collaborations, and publication purposes, translating complex scientific findings into accessible formats.• Provided advanced technical support during laboratory procedures, including troubleshooting equipment and protocol issues to minimize disruptions and ensure continuity.• Coordinated study- and project-related activities, contributing to cross-functional research efforts and fostering collaboration within multidisciplinary teams.• Independently designed and executed both qualitative and quantitative research studies, employing a range of methodologies such as molecular biology techniques, cell culture maintenance, confocal microscopy, flow cytometry, and high-throughput screening.• Managed laboratory resources efficiently by overseeing equipment stock, placing orders, and implementing inventory management systems to optimize laboratory workflows and reduce downtime.

• Supported research on the differentiation of induced pluripotent stem cells (iPSCs) into brain microvascular endothelial cells (BMECs), optimizing protocols to enhance cell yield and functionality.• Maintained iPSC and differentiated cell cultures, ensuring sterile conditions and monitoring growth characteristics.• Collaborated with graduate students to design and implement experiments focused on endothelial barrier properties and cellular resistance, providing technical support and sharing insights on methodologies.• Conducted data collection and analysis, utilizing statistical software to interpret experimental results and prepare comprehensive reports for lab meetings.• Maintained accurate laboratory records and assisted in managing supplies and compliance with safety regulations.• Stayed current with scientific literature to inform experimental designs and techniques, contributing to ongoing research discussions.

• Performed CRISPR gene editing, including transfection, single-cell dilution for obtaining targeted cell clones, and maintaining iPSC cell cultures for various research projects.• Assisted in the procurement, receipt, distribution, and inventory management of laboratory supplies and equipment, ensuring efficient laboratory operations.• Utilized PCR, TA Cloning, and DNA sequence analysis to confirm iPSC lines edited with CRISPR for multiple projects.• Employed SnapGene software to analyze CRISPR edits in cell clones, ensuring accuracy and precision in genetic modifications.• Prepared and dispatched samples for sequencing through Retrogen, facilitating the analysis of genetic modifications.• Conducted quality control assessments and maintained laboratory protocols to ensure experimental integrity and compliance with safety regulations.• Organized and maintained detailed electronic and physical records of experimental procedures, results, and observations to support data analysis and reproducibility.• Identified and resolved technical challenges in laboratory processes, enhancing efficiency and reliability of experimental outcomes.

• Optimized protocols to differentiate a H9 isogenic Huntington’s disease (HD) human embryonic stem cell derived (hESCs) series to brain microvascular endothelial cells (BMECs) to analyze dysregulation caused by mutant huntingtin protein (mHTT).• Utilized hydrogels to transition 2-dimensional BMEC cultures to a 3-dimensional environment to determine if mHTT expression influenced BMEC vessel formation and maturation.• Performed Journal Club sessions with mentor• Presented planned experiments to mentor and designed next experimental steps

- Rigorous program that teaches skills relating to Cell Culture, Immunohistochemistry, Q-PCR, Transfection, CRISPR and Western Blotting, and with many skills necessary for a successful internship at one of our collaborating institutions.- Opportunity for students aspiring to incorporate stem cell biology into their careers

• Utilized transfection methods to generate multiple mutant microfibril-associated glycoprotein 2 (MAGP2) in human embryonic kidney 293T (HEK293T) cells to test the ability to regulate TGF-β signaling.• Performed Western Blot and immunocytochemistry on generated mutated MAGP2 HEK293T cell lines to validate production of mutated MAGP2 protein production. • Maintained cultures of HEK293T for laboratory use.

• Scheduled and managed patient appointments across multiple departments, ensuring efficient use of clinic resources and optimizing patient access to healthcare services.• Provided excellent customer service by assisting patients with appointment inquiries, addressing concerns, and ensuring a positive experience.• Conducted system validation and data integrity checks to maintain accurate patient records, supporting compliance with healthcare regulations and privacy standards.• Collaborated with clinical and administrative teams to resolve scheduling conflicts and accommodate urgent requests, contributing to streamlined operations.• Trained staff on scheduling software and validation procedures, enhancing team efficiency and ensuring adherence to organizational protocols.• Utilized electronic health records (EPIC) systems to update patient information, verify insurance eligibility, and prepare documentation for appointments.• Identified and reported system issues, working with IT to troubleshoot and resolve technical problems that could impact patient scheduling and record accuracy.

• Prepared and served a variety of espresso, tea, and blended beverages while ensuring quality and consistency.• Delivered exceptional customer service, engaging with customers to create a welcoming environment and enhance their experience.• Managed cash transactions accurately and efficiently, handling point-of-sale (POS) operations and balancing cash drawers.• Maintained a clean and organized workspace, adhering to health and safety standards and ensuring a pleasant atmosphere for customers.• Assisted in inventory management, restocking supplies, and monitoring inventory levels to ensure efficient operations.• Collaborated with team members to optimize workflow during peak hours, maintaining high productivity and customer satisfaction.• Trained new employees on beverage preparation, store policies, and customer service protocols to support a cohesive team environment.