Muhammad Amit Khan Email & Phone Number

Dubai, United Arab Emirates

• Evaluated and processed Outpatient, Pharmacy and Inpatient prior authorization requests in accordance with policy’s schedule of benefits.• Evaluated the medical need for the requested services according to the medical data provided and accurately coded the service description codes according to accepted medical coding rules, medical guidelines and policy’s schedule of benefits.• Achieved proper capturing of the data on the system with a focus on accuracy, efficiency, and ensured that business decisions and processes are documented in a professional way.• Processed local and international reimbursement, resubmission and reconciliation claims by entering the accurate diagnosis and service codes and made sure that the payment calculation is in line with the policy terms and conditions.• Addressed member, broker and provider queries via calls and emails.• Managed high value OP and Pharmacy cases for cost containment and responsible for evaluating and escalating second medical opinion cases.• Reported suspected cases of fraud and/or abuse of both the members and healthcare providers to FWA team.• Responsible for training new joiners related to system usage, approval processes and reimbursement processes.

Oldenburg, Lower Saxony, Germany

Topic: Pathomechanisms and new therapeutic concepts in neuroinflammatory and neurodegenerative diseases.Abstract: Experimental autoimmune encephalomyelitis (EAE) is the commonly used model for studying Multiple sclerosis (MS). Here we demonstrate the right concentration of myelin oligodendrocyte glycoprotein (MOG) and mycobacterium tuberculosis (Mtb) that led to the induction of EAE in mice by immunization. α-MSH is an effective mediator in immunity and helps suppress inflammation, however, it is not chemically stable and prone to proteolytic degradation. Nle4-D-Phe7-α-melanocyte-stimulating hormone (NDP-MSH) on the other hand, is a more efficient and long-lasting synthetic analogue of α-MSH. Here we demonstrate, by means of immunofluorescence (IF) staining, the uptake of NDP-MSH-loaded microparticles into the draining organs such as lymph node (LN) and spleen as well as in liver. Additionally, it was found that the antigen presenting cells (APCs) were not responsible for the uptake of NDP-MSH-loaded microparticles. The process of isolation of murine brain microvascular endothelial cells (MbMECs) is crucial in studying the integrity of blood-brain barrier (BBB). Here we also demonstrate the successful isolation of brain endothelial cells that were then subjected to transendothelial electrical resistance (TEER) assay. It was found that NDP-MSH-treated groups had higher electrical resistance, which means improved integrity of the endothelial monolayer.Skills: Immunization of mice, measuring clinical score value, Immunohistology, cryo-sectioning, paraffin embedded sectioning, fluorescence microscopy, isolation of murine brain endothelial cells, induction of inflammation, measurement of transendothelial resistance, Microsoft Excel and GraphPad Prism.

Oldenburg, Lower Saxony, Germany

Tasks: Performing routine lab work including cell and molecular biology, and genetics techniques.

Oldenburg, Lower Saxony, Germany

Topic: Generating a RPGRORF15 isoform knockout in the hTERT RPE-1 cell line using the CRISPR/Cas9 systemAbstract: Retinitis Pigmentosa (RP) is a heterogenous group of inherited retinal dystrophies that affects 1 in 4000 people. RP is characterized by peripheral vision loss and ultimately complete blindness. It is caused by mutations in the RPGR (Retinitis Pigmentosa GTPase Regulator) gene. The purpose of this master thesis project was to generate a RPGRORF15 isoform knockout in the hTERT RPE-1 cell line. For this purpose, human female derived hTERT RPE-1 cell line was employed that already expressed all the three major RPGR isoforms. Five genotypically identical heterozygous clones were obtained via single cell sorting using FACS followed by PCR and sanger sequencing. Interestingly, we found that one of the alleles was completely deleted and the second allele acquired a single base pair insertion, leading to frameshift followed by premature stop codon, as verified at both genomic and transcript level. We observed that the RPGRORF15 knockout clones still expressed RPGRORF15 transcript with no change in the expression level, indicating that the transcript is not degraded by NMD pathway. We also confirmed that RPGRORF15 heterozygous knockout clones has an effect on the expression level of other isoforms RPGRex1-19 and RPGRskipΔ14/15, wherein the expression level of RPGRex1-19 is increased and the expression level of RPGRskipΔ14/15 is decreased. Characterization of RPGRORF15 knockouts at protein level further confirmed the expression of both RPGRex1-19 and RPGRskipΔ14/15 isoform proteins but not of RPGRORF15 isoform protein due to lack of RPGRORF15 positive control or RPGRORF15 specific antibody. Skills: CRISPR-Cas9 based transfection, Sanger Sequencing, FACS, manual cell dilution, Immortalized Cell Culture of HEK-293T and hTERT RPE-1 cell line, Freezing of cells, Western Blotting, Immunocytochemistry, DNA, RNA and Protein extraction and cDNA synthesis.

Oldenburg, Lower Saxony, Germany

Topic: Generating a RPGRORF15 isoform knockout in the hTERT RPE-1 cell line using the CRISPR/Cas9 system.Abstract: Primary cilia are the non-motile microtubular projections that are present in most of the cells in vertebrates. The primary function of the cilia is to serve as a skeletal and molecular pathway in the signal transduction processes such as mTOR, hedgehog Wnt, Notch, Hippo etc. Both mammalian photoreceptors of the eye, that is, Rods and Cones, contains specialized form of primary cilium called connecting cilium that is homologous to primary cilium both in function and structure. Any defect or change in the ciliary proteins can lead to the disruption of normal signalling pathway, thus leading to ciliopathies. One such ciliopathies is called Retinitis Pigmentosa. It is caused by the mutation in RPGR (Retinitis Pigmentosa GTPase Regulator) gene isoforms. There are three major RPGR isoforms, namely, RPGRex1-19, RPGRskipΔ14/15 and RPGRORF15. Any mutation in one of these RPGR isoforms may lead to Retinitis Pigmentosa. However, the complete function of RPGR gene and its isoforms are not fully understood. The purpose of this research project was to generate a RPGRORF15 isoform knockout in the hTERT RPE-1 cell line using the CRISPR/Cas9 system along with the dual guide RNA strategy. The project concluded with a successful cloning of sgRNAs into the eSpCas9(1.1) plasmid. Skills: Designing guide RNAs and Primers and off-target analysis using CHOPCHOP, Off-Spotter, UCSC In-silico PCR and SnapGene, DNA and RNA extraction, Polymerase Chain Reaction (PCR), Reverse transcription PCR (RT-PCR), UV spectrophotometry, agarose gel electrophoresis, gel imaging, Cloning, Sanger Sequencing, cell culture and Microscopy.

Dubai, United Arab Emirates

• Pre-authorized outpatient claims using “MedNext software” and “MedNet Connect” online portals, both for UAE and Oman policies, in line with medical codes (ICD-9 CM, ICD-10 CM, HCPCS and CPT codes) and international medical guidelines within set turnaround time. • Pre-authorized pharmacy requests via PBM (Pharmacy Benefit Management) and Openjet in line with medical policy terms and conditions. • Assisted in processing reimbursement claims by coordinating with the reimbursement, reconciliation, retrospective and finance team right from the level of Web and Mobile App submission, up to the level of fund settlement.• Assisted in processing paper and online claims by inserting medical codes and price rates as per agreed tariff. • Handled Members, Providers, International Partners, Brokers and Insurance queries related to inpatient, outpatient and pharmacy issues and provided legitimate solution as per terms and conditions.• Responsible for high end quality customer service and denial management across GCC countries and the rest of the world. • Responsible for orientation and training of new comers. • Responsible for continuous medical education via participating in different trainings and workshops.• Responsible for keeping up to date with the changing insurance policies related to DHA, MOH, HAAD and of other insurance partners on regular basis.• Reviewed high utilization medical trends and identified fraudulent cases and bringing them to the notice of Network and Medical Investigation Unit to minimize abuse of medical insurance policies.

Dubai, United Arab Emirates

• Responsible for pre-authorizing outpatient claims both for UAE and Oman policies, in line with health and medical insurance terms and conditions and within set turnaround time.• Responsible for pre-authorizing pharmacy requests both for UAE and Oman policies, in line with health and medical insurance terms and conditions and within set turnaround time.• Responsible for pre-authorizing inpatient, delivery and daycare surgery cases in case of emergencies in line with policy terms and conditions.• Responsible for providing top notch customer service to both international and local clients in terms of arranging appointments with medical providers, solving dispute if any between the patient and the medical provider and attending to any queries related to policy content and it's wordings.

Karachi, Pakistan

• Responsible for creating web-ready optimized content for our client websites.• Responsible for creating the title tags and meta-descriptions for medical content writing.• Responsible for Medical blog writing and Search engine optimization.• Liaised and coordinated effectively with both the internal and external clients to establish the projects assigned.

Karachi, Pakistan

• Processed claims by encoding medical diagnosis and procedures accurately to standard medical terminology using ICD-9 CM, HCPCS and CPT codes.• Maintained database for proceedings such as legal hearings and scheduling litigation hearings with defense attorneys and paralegals.• Liaised with U.S. based health insurance companies and insurance adjusters for the settlement and collection of pending medical claims (Workers Compensation Board).

Karāchi, Sindh, Pakistan

• Transcribed recorded dictations of all radiologic reports, general medical evaluation reports, medication follow-up reports, psychiatric evaluation reports, neurological consultation follow-up reports, new intake reports etc. of both American and Canadian Hospitals with 99% accuracy.• Distinguished between homonyms and recognized inconsistencies in medical terms by referring to dictionaries, drug references and other sources on Anatomy, Physiology and Medicine.• Returned dictated reports in printed or electronic form for physician’s review, signature and for inclusion in patient’s medical records.

Master'S Degree, Neuroscience, 1.61 Cgpa / A-Grade

Specialization: Human Genetics and Neuroimmmunology

Bachelor Of Science (B.S.), Human Physiology, 3.91 Cgpa / A-Grade

Specialization: Applied Physiology and Biophysics 1st Position holder