André Lapan Email & Phone Number

Metagenomic Discovery-Developed and ran assay to screen hundreds of novel enzymes across diverse biological families and identify several top candidates to improve Arbor's gene editing portfolio-Developed skills with Python to mine diverse metagenomic databases for proteins to screen. Wrote scripts to build out the downstream data analysis and condense vast amounts of data into useful formats for upward presentation and key decision making-Resulted in the discovery and patenting of several enzymes key to Arbor's portfolioLarge Integration-Built out several assays to quantify efficiency of large payload integration using novel gene therapy modalities and third-generation sequencing technologies-Used computational and ML-guided methods to engineer enzymes and improve efficiency of large cargo integration

NGS & Genomics team- Developed several comprehensive epigenetic assays (ChIP-Seq, ATAC-Seq) to determine the impact of gene editing on regulation of the CFTR gene- Streamlined and optimized NGS library prep for both Illumina short read systems and Nanopore long read technology- Cultured primary patient epitheleal cells and 16HBE cell lines for my team's assay development- Performed RT-qPCR and ddPCR assays for absolute quantification of spliced isoforms of CFTR

Focus on tissue engineering, medical device design, and the production of useful biomaterials from bacteriaSummer 2018-May 2019: Xiaocheng Jiang's LabSkills developed: Microbiology, bacterial culture, conductivity testing, biocompatibility testing, fluorescence imaging, SEM imaging, SolidWorks, laser cutter, 3D printing, spectrophotometry Contributions: Designed a device and protocol for the production of electrically conductive protein nanowires derived from electrochemically active bacteria for applications in biomedical sensingFall 2018: David Kaplan's LabSkills developed: Silk & collagen scaffold preparation, tissue engineering, mammalian cell culture, Alamar Blue, PicoGreen, LDH, & LIVE/DEAD viability assaysContributions: Constructed, cultured, and tested a 3D silk/collagen scaffold brain model to assess the effects of microglia on TBI inflammatory responseFall 2017: Fiorenzo Omenetto's LabSkills developed: Signal acquisition, data processing, MATLABContributions: Designed and tested silk RFID tattoos that monitor physiological markers in sweatFall 2014 - Spring 2015: David Kaplan's LabSkills developed: Tissue engineering, mammalian cell culture, microfabrication, photolithography, device design, ImageJContributions: Created and experimented with 3D human skin models to test wound healing devices which induce regeneration through external electrical stimulation

Boston

Volunteered 1700 hours over 10 months as a tutor, mentor, and role model for fifth-grade students at risk of failing or dropping out of inner-city underprivileged school. Developed lesson plans, built relationships with focus group students, tracked student attendance and presented schoolwide data, planned events to encourage student attendance, and communicated with family members. Developed leadership skills, interpersonal skills, resilience, and adapted to working with a dearth of support and resources.