Catherine Masiello Email & Phone Number

Cambridge, Massachusetts, United States

-Project lead on developing assays utilizing hybrid capture, NGS, ddPCR, and Jess Simple Western to improve protein expression and whole gene insertion activity of transposons-Performed mammalian cell transfections with flow cytometry, ddPCR, hybrid capture, and fluorescent microscopy readouts to assess proof-of-concept novel gene knock-in approach for large payload insertion at therapeutically relevant locus-Maintained multiple cell lines (HEK293T, N2a, AML12)-Maintained detailed and organized lab notebook, data files, and SOPs-Communicated research findings in cross-functional settings

Cambridge, Massachusetts, United States

-Screened novel nucleases for gene editing activity in vitro with NGS and gel electrophoresis-Improved nuclease editing activity by optimizing and screening gRNA designs in vitro and in mammalian cell lines-Maintained detailed and organized lab notebook, data files, and SOPs-Communicated research findings in cross-functional settings

Worcester, Massachusetts, United States

Shell Lab at WPI-Determine the impact of RBS sequence characteristics on M. smegmatis translation and mRNA stability using plasmids to transform modified RBS sequences into cell lines-Bioinformatic analysis of B11 loop complementary sequences in 5’ UTRs of M. abscessus genes, which was included in a research article pending publication-Continuation of EREE Fellowship project-Lab skills used: PCR, Gibson Assembly, gel electrophoresis, plasmid miniprep, DNA extraction, microscopy-Applications used: Microsoft Excel, GraphPad Prism, Python, Benchling, Artemis, FlowJo

Worcester, Massachusetts, United States

PLA for Molecular Biology

Worcester, Massachusetts, United States

Shell Lab at WPI-Bioinformatic analysis of 5’ UTRs of M. smegmatis and M. tuberculosis to identify characteristics (Shine-Dalgarno sequences, sequence motifs, sequence length, GA content) that could impact translation efficiency and mRNA stability-Applications used: Microsoft Excel, GraphPad Prism, MEME Suite, Weblogo, Python

Bachelor Of Science - Bs, Biology And Biotechnology

Activities and Societies: Masque, VOX, Alpha Psi Omega

Education record

Activities and Societies: Global and International Studies Program, Interact Club, Rose Players, International Thespian Society