Project: ”Effect of ultrasound on gating of ion channels” • Development of novel approach to simultaneously stimulate ion channels in artificial membranes by ultrasound and record channel gating at high temporal resolution. • Designed and executed project from conceptual idea to final experiment, led team of electrical engineers and biologists. • Expressed, purified and reconstituted functional mechanosensitive ion channel of large conductance (MscL) using E.coli expression system… Show more Project: ”Effect of ultrasound on gating of ion channels” • Development of novel approach to simultaneously stimulate ion channels in artificial membranes by ultrasound and record channel gating at high temporal resolution. • Designed and executed project from conceptual idea to final experiment, led team of electrical engineers and biologists. • Expressed, purified and reconstituted functional mechanosensitive ion channel of large conductance (MscL) using E.coli expression system, and 2-TM viral potassium channel using cell-free expression and simultaneous incorporation into lipid nanodiscs. Show less

At Columbia i am working in the Bioelectronics System Laboratory (Electrical Engineering) with Prof. Kenneth Shepard and in the Laboratory of Prof. Lars Dietrich (Biology). Project are at the intersection of Engineering and Biology with a focus on understanding protein function with novel tools.Project: ”Comparative functional characterisation of strain-specific SARS CoV E-proteins and involvement in host-specific virulence” • Concept to execution of multi-group overarching project… Show more At Columbia i am working in the Bioelectronics System Laboratory (Electrical Engineering) with Prof. Kenneth Shepard and in the Laboratory of Prof. Lars Dietrich (Biology). Project are at the intersection of Engineering and Biology with a focus on understanding protein function with novel tools.Project: ”Comparative functional characterisation of strain-specific SARS CoV E-proteins and involvement in host-specific virulence” • Concept to execution of multi-group overarching project aimed on functional characterisation CoV E- proteins. • Executed purification of SARS CoV2 E-proteins in cell-free expression systems. • Characterisation of E-proteins using single-channel recordings, western-blotting and mass photometry. • Leading efforts to identify FDA-approved compounds for drug repurposing to block E-protein.Project: ”Effect of ultrasound on gating of ion channels” • Development of novel approach to simultaneously stimulate ion channels in artificial membranes by ultrasound and record channel gating at high temporal resolution. • Designed and executed project from conceptual idea to final experiment, led team of electrical engineers and biologists. • Expressed, purified and reconstituted functional mechanosensitive ion channel of large conductance (MscL) using E.coli expression system, and 2-TM viral potassium channel using cell-free expression and simultaneous incorporation into lipid nanodiscs. Show less

Project: ”High-bandwidth recordings of ion-channel proteins using CMOS-suspended membranes” • Led an interdisciplinary collaboration between electrical engineers, material scientists, biologist and structural biologists. • Responsible for integration of artificial membrane systems with electronics and delivery of protein-of-interest to artificial membrane system. • Study extended temporal resolution of Type 1 Ryanodine Receptor recordings by two-orders of magnitude showing… Show more Project: ”High-bandwidth recordings of ion-channel proteins using CMOS-suspended membranes” • Led an interdisciplinary collaboration between electrical engineers, material scientists, biologist and structural biologists. • Responsible for integration of artificial membrane systems with electronics and delivery of protein-of-interest to artificial membrane system. • Study extended temporal resolution of Type 1 Ryanodine Receptor recordings by two-orders of magnitude showing experimental evidence of novel Calcium-binding site.Project: ”Development of screening platform for inhibitors of bacterial RND-efflux pumps”• Spearheaded pilot project towards the development of a novel screening technique for inhibitors of bacterial RND-efflux pump complex (three TM-proteins and one soluble protein).• Executed purification of RND-efflux pump complex consistent of four individual proteins. Proteins were expressed, purified and reconstituted in liposomes to provide functional characterisation of the complex in vitro.• Validation of complex formation using lipid nano-discs and various chromatographic methods.• Worked closely with electrical engineer to develop an electrochemical tool to study transport processes at RND-efflux pumps with extremely high sensitivity. Show less

We are developing next generation molecular diagnostic tools based on CMOS biosensors.