Andrew Lytle Email & Phone Number

Cell Culture; Assigned Full-Time 3-month contract at Rockland • Cell culture, cell counting and roller bottle production• Experience with diverse number of cell lines, adherent and suspension• Involvement with molecular team in cell-based assays and transfections• Culture of cells and preparation for ELISA testing• Freezing down vials and cryopreservation• Direct contact and coordination with several project leads• General lab cleanup and maintenance

Malvern, Pa

• Supported upstream and downstream process development of biologics, including purification studies, filtration, chromatography, centrifugation and cell culture techniques• Helped to develop and execute scalable strategies for improving downstream process performance through small-scale purification studies• Supported the transition from manual cell culture process to new cell culture technologies• Executed GMP and GLP experiment studies, authored and presented reports for said studies. • Performed data/graphical analyses using formulas and linking spreadsheets in Excel• Maintained and seeded mammalian cell culture lines, including passaging, harvesting, and other cell-based assays (cell counting, imaging)• Worked independently with no supervision, sought guidance when necessary• Performed reagent preparation, validation testing, and general laboratory maintenance• Documented detailed GMP and GLP research records within ELN electronic notebook• Conducted assays and lab procedures per testing records and regulatory guidelines• Served as cell culture trainer for two new hires

Millersville, Pa

• Awarded research grants, initiated and maintained zebrafish lab under Dr Cebra-Thomas and others• Developed training videos documenting In Situ Hybridization procedures (see YouTube channel ichthysseer) • Performed DNA cloning and plasmid DNA preparation, isolation• Conducted toxicology studies using zebrafish embryos: studied the effects of such things as Nicotine and Methional on the early development of Vertebrates• Experienced in zebrafish husbandry used for inheritance studies • Documented several years of experience keeping detailed lab notebook for molecular research

Millersville, Pa

Paid summer intern role within biology department• Conducted gene-expression studies of chondrichthyan dermal development• Presented poster at two scientific conferences (Millersville University exhibition and Annual Mid-Atlantic Developmental Biology Conference at Princeton)

Kansas City, Missouri Area

Fully paid summer intern for a large medical research institute with >$2billion in endowment • Summer Project: Analysis of gene expression in normal and disruptive mouse embryonic development• Successfully performed several in-situ hybridizations using numerous probes with wild-type and ENU- mutagenized embryos• Received training in GLP and other institute-related protocols• Trained in protocols necessary for RNA probe synthesis and in situ hybridization,• Handled the sacrifice of mice and dissection of mouse embryos• Documented stainings using laboratory-quality photography systems under high powered microscope