Contract Scientist in Clinical Next-Generation Sequencing Division: Product Support, Team Collaborations, Troubleshooting, Assay Development, Validation Studies, Molecular Biology

Molecular biology skills - DNA / RNA extraction, library preparationMicroarray genotyping of iPSC - characterization of stem cellsSOP / Protocol Management (Creating, editing, updating)Training junior personnel in sequencing, bead array, library preparationCardiomyocyte Cell CultureExperimental Design and Data ReportsDeadlines successfully met:Recruited 130 subjects for CARDiPS study between January and June 2013Sequencing projects and collaborations for production sequencing (2013-2014)Implemented organizational systems for stem cell and analyte storage (freezers and liquid nitrogen)Other tasks:Monthly reports of laboratory expendituresPerformed safety audits and served as lab manager

Analyze individual strengths and weaknesses in goaltenders, design drills accordingly, and provide advice for goalies of all ages.

Volunteer coach for Mite hockey team

Wrote case summaries, performed literature searches and prepared presentations, litigative support to other consultants. Work was primarily in environmental and industrial toxicology.

Performed experiments for PhD project as well as projects assigned within our lab.- DNA manipulation (purification, vector design, cloning, PCR)- protein manipulation (purification, blot, immunostaining)- Proficient in animal handling - mouse neurosurgery (performed intracranial stereotactic virus injection)- Tissue preparation and identification (tissue harvest, preparation, sectioning, colorimetric staining)- Grant and proposal writing (successfully acquired Ruth Kirschstein NIH grant in 2007)- Trained other employees (undergraduates, vet students, and other resarchers) in surgical procedures including animal handling, virus handling, and stereotactic injection- Graduate student instructor, Fall 2006. Held office hours, prepared and gave a lecture (Entitled "Protein Structure and Function"), wrote and graded exam question on said lecture.

Purified Factor VIII (Kogenate) by GMP guidelines, from product staging (acquire from fermentation dept) to product bagging (to send to filling dept).Performed all support functions including:- regeneration and equilibration of columns- all cleaning of skids, columns, tanks, and equipment- buffer creation- sample taking (water sampling as well as product sampling for QC)- training junior biotechnicians- writing safety audits