Anna York work email
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Anna York personal email
I am an Associate Research Scientist at the Yale School of Public Health. My work focuses on understanding the fundamental biology of Streptococcus pneumoniae, and to elucidate the molecular mechanisms that underpin important pneumococcal activities such as capsule switching or antibiotic resistance, with the aim to guide better prevention and treatments for disease caused by this bacterium. My PhD "A novel linkage between maintenance of protein translational fidelity and cell wall biosynthesis in Streptococcus pneumoniae" focused on MurM, a cytoplasmic protein which is important for the development of high-level penicillin resistance. My postdoctoral work focused on developing a method for enrichment of pneumococcus from a mixed serotype sample (in lab experiments), or a polymicrobial sample (such as saliva), which makes isolation of serotype specific pneumococci more efficient. My current research as an Associate Research Scientist focuses on the capsular polysaccharide, and gaining a more complete understanding genetic and metabolic restrictions for pneumococcal capsule switching and the subsequent impact of this on the emergence of new serotypes following vaccine implementation.Since joining Yale in 2019 I have been heavily involved in the Yale Postdoctoral Association (YPA), holding leadership positions for Professional Development Committee, Symposium Committee and the Treasury, as well as being elected co-chair of the association from 2021-2022. I have a keen interest in science communication and public engagement, and enjoy mentoring students in the lab.
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PhdUniversity Of Warwick Sep 2014 - Mar 2019 -
Mini Project 2 - Kinetic Investigations Of Substrate Analogues Of S.Pneumoniae And P.Aeruginosa MurfUniversity Of Warwick Apr 2015 - Jun 2015School Of Life SciencesMy second mini-project was focused on the structure activity relationship of MurF substrate analogues, with the aim to develop a ‘torjan-horse’ antimicrobial compound. MurF was expressed and purified using a combination of IMAC, SEC and ion exchange chromatography. Michaelis-Menten kinetics of the natural substrate and five structural substrate analogues were determined using simple coupled spectrophotometric assays. All analogues were suitable substrates for the MurF enzyme, however the catalytic efficiency was considerably lower that that of the natural substrate. Crystallisation trials were conducted, aimed at obtaining structural information about MurF in complex with the substrate analogues for iterative structural analogue design. -
Pip - Community And Education Development OfficerCawthron Institute Jul 2015 - Sep 2015Nelson, New ZealandMy Professional Internship for PhD Students (PIPS) was a three month project at The Cawthron Institute in New Zealand. This was a unique opportunity to gain insight into the importance of science communication and education within the wider community. Skills required for communicating science to the general public as well as specialist audiences were developed and refined. I was interviewed at a local radio station and my scientific presentation on “The antibiotic apocalypse” was videoed, these two unique opportunities enabled me to analyse and reflect on and enhance my presentation techniques as well as gain a better insight into how best to communicate my science to different audiences. A knowledge of programming in BASIC was used to create a versatile programme for the Community and Education Department to assist in the allocation of students, and judges at a large annual Science Fair in New Zealand, the programme was created with a variety of automated outputs including timetables and acceptance letters. -
Purification And Crystal Screening Of Mglabd-Bd2492, Bd2492 And Romr MutantsUniversity Of Birmingham Jan 2015 - Mar 2015School Of BiosciencesMy first mini-project was to conduct structural characterisation of proteins believed to be involved in control of predation in the deltaproteobacteria Bdellovibrio bacteriovorus. A combination of molecular biology techniques including cloning and PCR were used to generate mutant protein constructs. Proteins were overexpressed and purified using a combination of Immobilised Metal Affinity Chromatography (IMAC) and Size Exclusion Chromatography (SEC). Crystallisation trials were conducted on the MglABd-Bd2592 complex as well as RomR and Bd2492 individually, crystallisation conditions were subsequently optimised, however, diffraction-quality crystals were not obtained. -
Undergraduate Dissertation - Investigating Interactions Between Auxilin And Its Binding PartnersUniversity Of Warwick May 2014 - Jun 2014School Of Life SciencesAn investigation into the interactions between auxillin and its binding partners, clathrin and Hsc70, using fluorescently labelled single cysteine mutants was conducted. Real-time perpendicular light scattering was used to demonstrate that fluorescently labelled mutants of clathrin and Hsc70 did not affect disassembly of clathrin cages. In a novel study, the potential for use of time-resolved fluorecence anisotropy in understanding clathrin cage dissassembly was investigated. The proof of principal study found that time-resolved fluorescence anisotrpic changes were consistent with current understanding of the system and could hold unexplored promise and potential for understanding disassembly at a molecular level. -
Industrial Placement StudentGlaxosmithkline Pharma Gmbh Jun 2012 - Jun 2013Stevenage, Hertfordshire, United KingdomGeneration of tool monoclonal antibodies towards oncogenic transcription factors L-MYC and C-MYC. Techniques required for antibody generation included preparation of mouse splenocytes and fusion with myelomas, sterile mammalian cell culture techniques, colony screening using ClonePix system, Flow Cytometry (FACS analysis) and high throughput sample preparation with robotics, and purification techniques using AKTA. Antibodies were validated using a range of techniques specific to the request of the researcher, often including ELISA, FMAT and Western blot.
Anna York Education Details
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Molecular Biochemistry
Frequently Asked Questions about Anna York
What is Anna York's role at the current company?
Anna York's current role is Associate Research Scientist at Yale School of Public Health.
What is Anna York's email address?
Anna York's email address is a.****@****k.ac.uk
What schools did Anna York attend?
Anna York attended University Of Warwick, University Of Warwick.
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Anna Lofton
General Counsel At Central Pension Fund Of The Iuoe & Participating EmployersWashington, Dc1cpfiuoe.org
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