Annis-Rayan Bourefis Email & Phone Number

Toronto, Ontario, Canada

Ville De Paris, Île-De-France, France

Development of web applications providing management and recruitment tools for health personnel:• Initiation and planification of multiple projects in parallel in accordance with user needs• Development of new modules, code refactoring and test coverage• Virtual machine implementation and deployment operations• Time and cost estimation• Active meeting participation and animation (Agile organisation)

Ville De Paris, Île-De-France, France

3-month Full Stack Web Developer internship focusing on Java/JEE and Angular technologies:• Final project: creation of a website for wildlife monitoring

Ville De Paris, Île-De-France, France

The objective was to investigate the pathogenic mechanisms induced in Amyotrophic Lateral Sclerosis (ALS), a neurodegenerative disorder, by two genes: FUS, implicted in RNA metabolism, and CHCHD10, involved in mitochondria stability.To do this, we characterized two novel stable non-sense mutant zebrafish models for the orthologues of these genes and highlighted several ALS phenotypic features:reduced lifespan, locomotor disabilities, aberrant motor axons, disorganized neuromuscular junctions, altered muscle morphology and disrupted mitochondrial network, as well as RNA and protein expression changes of the protein tau, involved in Fronto-temporal Dementia and other tauopathies, and kinases acting on tau phosphorylation.Laboratory activities:- Biomolecular procedures: Western Blots, qPCR, genotyping, sequencing- Immunohistochemistry- In vivo and in vitro confocal imaging- 3D reconstruction analysis (Imaris Software)- Micro-injections at zebrafish embryonic stage- Drug screening- Behavioral assessments: locomotion, light sensitivity- Survival assays- Statistical analyses

Ville De Paris, Île-De-France, France

The objective was to investigate the pathogenic mechanisms induced in Amyotrophic Lateral Sclerosis (ALS), a neurodegenerative disorder, by two genes: FUS, implicted in RNA metabolism, and CHCHD10, involved in mitochondria stability.To do this, we characterized two novel stable non-sense mutant zebrafish models for the orthologues of these genes and highlighted several ALS phenotypic features:reduced lifespan, locomotor disabilities, aberrant motor axons, disorganized neuromuscular junctions, altered muscle morphology and disrupted mitochondrial network, as well as RNA and protein expression changes of the protein tau, involved in Fronto-temporal Dementia and other tauopathies, and kinases acting on tau phosphorylation.Laboratory activities:- Biomolecular procedures: Western Blots, qPCR, genotyping, sequencing- Immunohistochemistry- In vivo and in vitro confocal imaging- 3D reconstruction analysis (Imaris Software)- Micro-injections at zebrafish embryonic stage- Drug screening- Behavioral assessments: locomotion, light sensitivity- Survival assays- Statistical analyses

Paris, France

Internship on the effect of age on visual information encoding in humans during a spatial navigation task.Laboratory activities: eye-tracking and motion capture system, behavioural tests on humans.

Bristol, Royaume-Uni

Internship on the development of genetic models of Down Syndrome in drosophila.Laboratory activities: genetic manipulations, learning and memory tests, locomotion tests.

Paris, France

Internship on inflammatory myopathies.Laboratory activities: cell culture (primary cells and cell lines), cellular labelling, flow cytometry (FACS), animal handling (mouse).

Master Of Science (Msc), Integrative Biology, Neurosciences

Relevant coursework: - Integrative Neurophysiology - Physiological and pathological neurotransmission and signalisation - Animal models.

Bachelor Of Science (Bsc), Life Sciences, Biology, General

Relevant coursework: - Functional Integration of Hormonal and Neuronal Signals - Functional Neurobiology - Cell and Development Biology.