Arpit Mishra, Phd Email & Phone Number

*An experienced hybrid biomedical scientist with a demonstrated history of working in a professional and academic setting for over 8 years. #Functional Validation of Autoimmune Disease enhancer variants. We are using MPRA, ATAC-seq and CRISPR to determine allelic difference in enhancer activity using primary human T cells.#Investigation of 3D Genome Architecture to Map Enhancer-target Genes.  We are using capture Hi-C to determine the target genes of enhancers of interests from primary human T cells.#Current/past projects #Structure-functional studies, biophysics of small molecule-DNA interactions, #protein purification and activity optimization for novel high fidelity Cas9 enzymes for genome editing, optimizing RNP approaches for genome editing, #Off target CRISPR integration and on target analysis for Adenovirus mediated CRISPR edits using CRISPR NGS libraries# single cell RNA seq experiments using combinatorial indexing approach and data analysis using scanpy Key Skills: Benchwork: Functional genomics using ChIP seq, RNA-Seq, Sc RNA-Seq (droplet and combinatorial indexing based approach), Hi-C, Capture HiC, MPRA, STARR-SEQ, CRISPR screens , On target off target library preparation, library sequencing using illumina platform (Nextseq 550)Computational work:NGS data analysis for RNA-Seq, Sc RNA-Seq, HI-C, Capture Hi-C, CRISPR off target on target analysis, and data visualization using Shell scripting, R and Python packages, version control with GIT, (https://github.com/bioarpit1)

Listed skills include Molecular Biology, Pcr, Sds Page, Biochemistry, and 37 others.