Ashly Lucas Email & Phone Number

Seattle, Washington, United States

Seattle, Washington, United States

Seattle, Washington, United States

Seattle, Washington, United States

Greater Seattle Area

-Contributed to authoring sections of a BLA filing, lead data verification efforts, and contributed to responses to FDA IR letters. -Multi-color flow assay development and Lifecycle management for both product characterization and release testing using a QBD approach-Extensive statistics based flow analysis (jmp)-Flow Cytometry Trainer/SME for primary T-cell characterization method

Thousand Oaks, Ca

-Developed multi-color flow cytometry assays for allogeneic TCR-T and CAR-T cells.-Qualified ELISAs for process related impurities and residuals analysis-Supported lab infrastructure; including sample management, maintaining reagent and consumables inventories, and data management.-Expansion, maintenance, and banking of primary cells and undifferentiated cell lines-Karyotyping and HLA typing-Standardizing cytometers across labs

Greater Los Angeles Area

-CAR T-Cell Therapy manufacturing in a cGMP ISO 7 cleanroom environment

-Maintenance, expansion, and banking of various mammalian mesenchymal stem cells.-Isolating epithelial, endothelial, and mesenchymal stem cells from tissue for expansion and long term cryopreservation.-Developed and qualified cell-based assays including proliferation, luciferase based, species identification assay(PCR), differentiation, and ELISAs for cytokine detection and residuals. -Routinely ran multiplex Luminex assays-Assisted in the development of a qPCR assay for gene expression in feline cell lines.-Worked on a team that developed a process for large scale expansion of MSCs for use in veterinary cell therapies.-Maintained flow cytometers.

Developing methods to observe and detect cytotoxicity of p. aeruginosa in CFBE cultures-Assisting with the generation of knock-out cell lines to determine a gene's role in p.aeruginosa cytotoxicity and biofilm formation. -Heavy use of imaging assays to detect caspase activity with bright field microscopy.