Aster Escalante Email & Phone Number

May 2024 -Joined Hamilton as Field Applications Specialist

La Verne, California, United States

● Developed and executed sales and distribution strategies resulting in a 4X increase in external revenue.● Implemented standard operating procedures for account management, marketing, forecasting, distribution.● Worked closely with Production to determine product allocations for external sales accounts.● Secured approval for 5 product SKUs at all Total Wine & More locations in California within five months.

Claremont, California, United States

● Established proof of concept for cell-free protein expression with translocation across proteolipid bilayer.● Executed assays: cell-free protein expression, DHFR, Western Blot of Proteinase K-digested samples.● Purified key translocon from E. coli through affinity chromatography on Akta Pure system. ● Assembled and optimized supported lipid bilayers within in-house 3-D printed PLA cartridges.

Claremont, Ca

● Designed and executed lesson plans for mammalian cell culture and microbial fermentation courses. ● Provided training in aseptic technique, bioreactor assembly & operation, media preparation, data analytics.● Managed Team Master's Projects assessing media optimization for CHO cells expressing monoclonal IgG.

Claremont, California, United States

● Led a sponsored research project assessing the comparability of HyPerforma G3Labs and GXCore Cell Culture biocontrollers utilizing the Emerson Delta V operating system.● Managed and trained graduate student team on aseptic technique, media preparation, mammalian cell culture, bioreactor assembly, biocontroller operation, and relevant data analytics.● Presented comparability data and conclusions to external stakeholders and provided a summary report.

Claremont, California, United States

● Optimized fermentation conditions for Pichia pastoris expressing insulin under constitutive GAP promoter. ● Characterized purification of insulin lispro using inorganic solvents, detected via Western blot assay.● Organized and tracked laboratory inventory, equipment use and submitted purchase orders as needed.● Trained students and visiting researchers on molecular biology, cell culture and fermentation techniques.

Claremont, California, United States

Sponsored research project in collaboration with Thermo Fisher Scientific and Applikon Biotechnology● Collaborated in a student team characterizing a mini-scale bioreactor for recombinant mammalian cells. ● Led and performed CHO cell culture studies in fed-batch mode under variable P/V parameters.

Claremont, California, United States

Sponsored research project in collaboration with Bristol-Myers Squibb● Collaborated in a student team on the methodology for cell culture scale-down model development. ● Conducted mammalian cell bioreactor studies implementing key scale-down parameters derived from literature research and subject matter expert interviews.● Developed and managed project budget plan, including estimations for materials, equipment, and travel.● Tracked project expenses, ensured adherence to the budget plan, and promptly addressed any deviations.

Master Of Business And Science (Mbs), Bioprocessing

Activities and Societies: KGI-ISPE Student Chapter Secretary, Budget OfficerTeam Masters Project: Demonstrating Process Scalability.

Bachelor Of Science (B.S.), Biology

Activities and Societies: Maximizing Access to Research Careers - Undergraduate Student Training in Academic Research (MARC U*) California.

Associate Of Arts (A.A.), Humanities; Arts And Social Sciences; Social And Behavioral Sciences

Activities and Societies: Anthropology ClubParticipated in fundraising events and outdoor excursions to learn about local Native American.

Associate Of Science (A.S.), Science And Mathematics

General education courses as well as introductory science courses in biology, chemistry, physics, anatomy & physiology.