Alexei V. Medvedev, Phd Email & Phone Number

Washington D.C. Metro Area

Research, develop, and produce Rubella-based viral vectors to deliver custom gene therapy products inside cells to elicit an adaptive immune response to a wide range of infectious disease pathogens, including Human Immunodeficiency Virus (HIV), Plasmodium falciparum (Malaria infectious agent), Rabies virus, and other future targets.Explore potential cancer vaccine development targeting production of neutralizing monoclonal antibodies that would direct cytotoxic effects to a tumor… Show more Research, develop, and produce Rubella-based viral vectors to deliver custom gene therapy products inside cells to elicit an adaptive immune response to a wide range of infectious disease pathogens, including Human Immunodeficiency Virus (HIV), Plasmodium falciparum (Malaria infectious agent), Rabies virus, and other future targets.Explore potential cancer vaccine development targeting production of neutralizing monoclonal antibodies that would direct cytotoxic effects to a tumor cell.Advance practical experience in microbiology and immunology research setting by attaining a mastery of cell culture techniques, including mammalian cell culture maintenance, transfection of genetic material into cells, confirmation and analysis of custom gene expression inside cells, extraction and sequencing of cellular genetic material during post-production phase of vaccine manufacturing process, and candidate vaccine vector quantification to ensure identity, purity, potency and consistency of production.Adapt computer simulation approaches developed during graduate work to model theoretical protein structures of target pathogen components to assess their effectiveness in serving as a basis for improved vaccine design.Apply viral vectors as a functional cure for HIV and cancer.Continue professional growth by attending department lectures and seminars to stay abreast of latest scientific theories, experiments, and technology trends that may have bearing upon the development of viral gene vectors for vaccine applications. Show less

Washington D.C. Metro Area

Examined genome replication of human Norovirus (hNoV), a potent infectious disease agent, by focusing on the role that VPg plays in the initiation of genomic and sub-genomic RNA synthesis by hNoV polymerase (RdRp) and its polymerase-protease precursor protein (ProPol).Manufactured and characterized proteins by genetically engineering and maintaining plasmids required for protein expression, producing desired proteins in bacterial culture, establishing identity and purity of synthesized… Show more Examined genome replication of human Norovirus (hNoV), a potent infectious disease agent, by focusing on the role that VPg plays in the initiation of genomic and sub-genomic RNA synthesis by hNoV polymerase (RdRp) and its polymerase-protease precursor protein (ProPol).Manufactured and characterized proteins by genetically engineering and maintaining plasmids required for protein expression, producing desired proteins in bacterial culture, establishing identity and purity of synthesized proteins, determining protein potency, and reproducing experiments to ensure consistency of protein products.Enhanced the performance of the hNoV VPg nucleotidylylation assay 100-fold by developing and applying a modernized technical approach to the protein manufacturing process, leading to higher potency reagents and a resultant demonstration of ProPol’s likely role as the primary polymerase responsible for hNoV VPg nucleotidylylation.Identified the requirements for, initiated, planned, and executed the development of a computer modeling protocol to simulate predicted protein structure that resulted in a discovery of nucleotide triphosphate binding capacity of the hNoV VPg and the involvement of VPg’s charged N-terminal amino acids in that binding process.Developed and applied a biomarker assay for Hepatocellular Carcinoma in collaboration with an interdisciplinary team of scientists and physicians at Georgetown University Lombardi Cancer Center.Led a group of rotating undergraduate and graduate students by mentoring them in modern laboratory standard concepts, practices, and procedures within the fields of microbiology, molecular biology, and immunology. Maintained written communication with experts and peers in the field of Norovirus research.Honed oral communication skills by presenting research findings to faculty and students at department seminars, as well as poster presentations at conferences.Managed day-to-day laboratory operations. Show less

Greater Milwaukee Area

Studied protein localization in Pseudomonas aeruginosa bacterium.Researched and developed protocols for cell separation using differential centrifugation and filtration methods to collect minicells enriched in polar proteins.

Madison, Wisconsin Area

Selected to participate in the Summer Public Health Fellowship.Developed advanced techniques for internet research and analysis of epidemiological data related to smokeless tobacco usage and advertising on Web 2.0 sites.Independently researched open source software and technologies to develop and apply new technical approaches to gather and study relevant information for behavioral analysis of smokeless tobacco consumers, including demographic data, user presence and activity on… Show more Selected to participate in the Summer Public Health Fellowship.Developed advanced techniques for internet research and analysis of epidemiological data related to smokeless tobacco usage and advertising on Web 2.0 sites.Independently researched open source software and technologies to develop and apply new technical approaches to gather and study relevant information for behavioral analysis of smokeless tobacco consumers, including demographic data, user presence and activity on social networking sites, as well as subject ties to smokeless tobacco industry. Produced and implemented an operations manual to assess behavioral patterns within the collected data. Show less

Greater Milwaukee Area

Managed online and phone sales, as well as technical support for current and prospective customers at an online home mortgage pricing start-up company.Interacted with prospective and current clients, supervisors, members of the mortgage industry, as well as company sponsors and stakeholders. Served as an intermediary in direct contact with software users and technology developers.Structured company website for organic search engine optimization and pay-per-click… Show more Managed online and phone sales, as well as technical support for current and prospective customers at an online home mortgage pricing start-up company.Interacted with prospective and current clients, supervisors, members of the mortgage industry, as well as company sponsors and stakeholders. Served as an intermediary in direct contact with software users and technology developers.Structured company website for organic search engine optimization and pay-per-click campaigns, including Google AdWords, Microsoft adCenter, and Yahoo! Search Marketing services.Performed entry-level software development using company’s proprietary scripting language to produce loan quotation databases that assisted mortgage brokers in pricing home loans for their customers.Conducted literature searches, as well as collected published information and reports, to develop and produce educational web articles about the secondary loan market. Show less

Madison, Wisconsin Area

Researched, developed, and executed novel laboratory experimental methods for examining Drosophila melanogaster responsiveness to sensory stimuli including light, touch, and various chemical compounds.Determined the impact of method improvements, evaluated effectiveness of the experimental strategies and tactics, and published findings in a co-authored publication. Maintained Drosophila stocks and mated flies to achieve desirable phenotypes for research.

Greater Milwaukee Area

Selected to participate in the elite Summer Program for Undergraduate Research (SPUR).Studied protein localization in Saccharomyces cerevisiae yeast.

Doctor Of Philosophy (Ph.D.), Microbiology

Dissertation topic: Characterization of VPg nucleotidylylation and nucleoside triphosphate binding in Norovirus, a single-stranded.

Non-Degree Graduate Student, Molecular Biology Of Microorganisms

Continued education by successfully completing a graduate-level Molecular Biology of Microorganisms course (Bio Sci 529) that provided.

Education record

Achieved over 50 semester hours of basic and advanced medical science coursework, leading to a mastery of topics including, but not.

Bs, Genetics

Fulfilled 27 semester hours in biological sciences with coursework in biology, microbiology, as well as genetics. Completed 39 semester.