Lab manager, molecular biologist.

Manage research lab, perform molecular biology experiments.

• Planned animal experiment with animal surgery tech for Deep Vein Thrombosis (DVT), and treatment with nanoparticles conjugated with various antithrombin drugs to compare the size of thrombus in treated and control animals.• Performed various molecular biology techniques like western blot, ELISA, IHC and H & E staining on treated animal tissues and plasma.• Performed statistical analysis, helped in writing grants, posters and research articles.• Trained graduate and medical students.• Maintain lab and lab equipments, ordered supplies.

• Extracted RNA from human vascular tissue and Smooth Muscle cells, mouse abdominal aortic aneurysm and blood.• Quantified and qualified RNA through the use of the Agilent Bioanalyzer 2100• RT-PCR, operated ABI Fast7500 instrument• Performed protein detection by Western blot, Qaigen Luminax Liquichip and ELISA• Established cell culture and maintained the primary culture of Smooth Muscle Cells obtained from human vascular tissues• Performed immunofluorescence Labeling of Cultured Cells • Executed Flow Cytometry for biomarker detection and cell characterization on FACSCalibur • Operated FlowJo software for FACS analysis • Immunohistochemistry and H & E and VVG staining• Maintained lab equipment and inventory stocks• Trained residents, fellows and research collaborators on molecular biology skills and responsible laboratory practices• Performed Radioactive Elastolytic Assays• Facilitated collaboration with other labs on major experiments