Ben Brophy Email & Phone Number

Davis, California, United States

Collaborates with a project scientist to study the unclear mechanism behind the transition between epigenetic states at a given locus in the genome to improve epigenetic editing tools for persistent gene silencing. -Maintains various cell lines and regularly performs transfections and different treatments on various cell lines -Carries out RNA Preps, Reverse Transcription, qPCR, and FACS from treated cell line samples -Designs primers to be used for various activities -Preps plates for culturing iPSC derived neurons-Frequently clones different gRNAs for usage in treatments and other applications-Extracts RNA from various mice tissue samples

Davis, California, United States

Assisted the R&D Microbiology and Plant Sciences Department on various projects relating to products in development, such as bio-fungicides and bioinsecticides. -Maintained various, ample, and weekly fungal and bacterial cultures -Performed DNA and RNA extractions on leaf and root collections of different plants -Used BCA Assays coupled with Protein Extractions to screen for a particular metabolite -Kept a steady collection of cucumber plants

Baltimore, Maryland, United States

Collaborated with a postdoc in Dr. Haig Kazazian's Lab to detect and study L1 retrotransposons within the single nuclei of normal, cancerous, and metastasized tissue of patients with various cancers.➤ Performed DNA Plasmid Extractions and Extractions of DNA from cell culture➤ Analyzed samples using Qubit and NanoDrop➤ Genotyped and handled mice➤ Assisted in BCA Assays and harvesting cells undergoing gene knockout via CRISPR/Cas9 ➤ Recorded data and results in an organized fashion(Unpaid Internship)