Beth Chang Email & Phone Number

Raulet Lab

• My project was to examine the cellular mechanisms of MHC Class I-deficient tumor clearance in response to intratumoral injection of cyclic di-nucleotides• Assessed Natural Killer (NK) cell activation in tumor-bearing mice by flow cytometry (IFNg, GzmB, Ki67, Sca1, and CD107) • Performed chromium-51 killing assays with NK cells taken ex vivo on tumor targets• Examined tumor-specific T cell function using peptide stimulation assays followed by intracellular cytokine staining and ELISAs of supernatants• Experienced at handling mice – taking ear clippings and blood for genotyping and tumor measurements with calipers• Supervised another undergraduate research assistant on experiments including flow cytometry, PCR, sequencing, and mouse tissue harvesting

• Volunteer as a Mandarin interpreter for English proficiency limited Mandarin speaking patients at health clinics including SAHA blood pressure clinic, Chinatown health fair, and online health-related documents translation for a total of 10 hours each semester• Lead language portion of the interpretation training course for new members, giving weekly quizzes, preparing and giving lectures on medical/health-related keywords, and conducting interpretation practices as the Mandarin Language Coach for Fall 2018 semester

Tainan City, Taiwan

• Participated in a project for prostate cancer DNA-based vaccine development using prostate specific antigen (PSA) on prostate cancer cells as a potential antigen for the DNA vaccine• Assisted with the preparation of developed vaccine for animal (mice) experiment• Learned basic lab skills including PCR sequencing with modified DNA plasmid, cell culturing, and fluorescence imaging