Brian Engel Email and Phone Number
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Brian Engel is a Senior Development Scientist at Beckman Coulter Diagnostics. He possess expertise in biochemistry, molecular biology, cell culture, microscopy, western blotting and 23 more skills.
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Senior Development ScientistBeckman Coulter DiagnosticsMinneapolis, Mn, Us
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Senior Development ScientistBeckman Coulter Diagnostics Jan 2022 - PresentChaska, Minnesota, United States -
InstructorMd Anderson Cancer Center Feb 2021 - Jan 2022Houston, Texas, United States•Engineered macrocyclic peptide ligands to improve target affinity and inhibition of autophagy in ovarian cancer. •Employed mRNA Display directed evolution to generate nanomolar ligands for cancer-associated antigens. •Leveraged Illumina sequencing to characterize directed evolution DNA libraries. •Evaluated apoptosis and inflammation PET radiotracers as early indicators of liver disease in mouse models. -
Postdoctoral FellowMd Anderson Cancer Center Feb 2016 - Feb 2021Houston, Texas Area•Developed peptide agents for non-invasive imaging of cell death in vitro (fluorescence assays, confocal microscopy) and in vivo (liver toxicity and ovarian cancer mouse models).•Characterized immune checkpoint receptor ligands (flow cytometry and confocal microscopy).•Performed mRNA Display directed evolution including cloning, DNA library assembly, in vitro transcription, in vitro translation, post-translational modification and affinity capture of oligonucleotides, peptides and proteins. •Synthesized and characterized lead candidates for binding and stability (HPLC, LC-MS, FP).•Expressed and purified recombinant enzymes or antigens using affinity, ion exchange, or size exclusion chromatography. •Characterized recombinant proteins with binding and activity assays (ELISA, FP, SDS-PAGE, western blot, enzyme assays). •Radiolabeled peptides and small molecules for small animal PET/CT & PET/MRI to assess target binding and pharmacokinetics. •Performed preventative maintenance, repairs, training and acquisition of laboratory equipment including HPLCs, LC-MS, lyophilizers, rotary evaporators, gamma counters, scintillation counters and flow cytometers.
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Phd Candidate, Department Of BiosciencesRice University Aug 2010 - Dec 2015Houston, Texas•Developed high throughput/high content 3D hydrogel cell culture models with automated image analysis for improved in vitro prediction of in vivo drug efficacy. •Investigated role of mucin glycoproteins on the transcriptional regulation of EGFR and the physiological effects of that regulation (qPCR, ChIP, luciferase promoter assays, western blot, CRISPR/cas9, MTS, BrdU, colony forming assays, drug sensitivity). •Established ex vivo 3D hydrogel cell culture of endometrial patient-derived xenografts. -
Lab Analyst I And IiPace Analytical Sep 2007 - Jul 2010St. Paul, MnWorked with a cross-disciplinary team to develop the 3M™ Attest™ Super Rapid Readout Biological Indicator product line. -
Undergraduate Research AssistantUniversity Of Minnesota Apr 2005 - Aug 2007St. Paul, MnDeveloped and tested hydrogen-producing bacterial microphotobioreactors.
Brian Engel Skills
Brian Engel Education Details
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Biochemistry And Cell Biology
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Biochemistry
Frequently Asked Questions about Brian Engel
What company does Brian Engel work for?
Brian Engel works for Beckman Coulter Diagnostics
What is Brian Engel's role at the current company?
Brian Engel's current role is Senior Development Scientist.
What is Brian Engel's email address?
Brian Engel's email address is br****@****ing.org
What is Brian Engel's direct phone number?
Brian Engel's direct phone number is (713) 348*****
What schools did Brian Engel attend?
Brian Engel attended Rice University, University Of Minnesota-Twin Cities.
What skills is Brian Engel known for?
Brian Engel has skills like Biochemistry, Molecular Biology, Cell Culture, Microscopy, Western Blotting, Biotechnology, Laboratory, Lifesciences, Pcr, Biology, Fluorescence Microscopy, Research.
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Brian Engel
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Brian Engel
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