A total of two manuscripts edited

Duties within project:-Development of a mosquito larvicide based on yeast-encapsulated essential oils (international consortium managed by Ivy Hurtwitz, UNM, USA and initially funded by the Centers for Disease Control and Prevention Foundation - grant number 200-2017-93140).-I was the medical entomology & Scientist responsible for the experiment to evaluate larvicide efficiency of yeast-encapsulated essential oils in Brazil (IOC/Fiocruz) that include experiements under laboratory to test inseticide susceptibility (multiple lineages of Aedes aegypti, and Aedes albopictus), semi-field to verify product stability/activity across time (Aedes aegypti Rockefeller) and field trials to evaluate larvicide activity and breeding site recovery with mosquito ID (multiple mosquito species). Moreover, we also carry bioecology assays to understand other impacts of the product (e.g. impact on mosquito oviposition).-As project manager, I was part of the team responsible to establish the communication between Fiocruz (BR) and UNM (US), manage the budget required for higher technical staff and comsumables and equipment in Brazil, submit regular reports about the scientific progress of the project.-I was responsable to supervise and support all technical staff and students involved in the project, which require working with students from different regions of Brazil (Minas Gerais, Pará).Duties outside the main project-I also participate as Teacher/coordinator/co-supervisor in the Professional Master’s in Vector Control and Surveillance that aim to reinforce the knowledge about vector-borne diseases of persons working on vector control in Brazil.-I provide support on other activities of my laboratory (LABFISI): Innovation liaison, supervision of students with different academic background, courses coordination, funding, science communication activities and science-art activities.

A total of 62 manuscripts edited

I participate in two different projects:1)The project aim to The study of molecular tools for insecticide resistance diagnosis in phlebotomine sandflies for sustainable leishmaniasis control and elimination programmes, and it was funded by by LSTM internal ISSF Challenge Grant Application. -Optimization of molecular tools to genotype Kdr-1014 (e.g. PCR, SANGER sequencing, TaqMan SNPs identification) in LSTM (UK) facilities.-In India, I carried insectary experiments and prepare samples for molecular biology with F1 and colony sand flies: i) WHO assays with multiples insecticides (DDT and pyrethroids), ii) RNA extraction of sand flies pools, iii) preparation of RNA samples for shipment/transportation- Preparation of samples for RNA-sequencing by llumina HiSeq4000 (Paired-end, 2x150 bp sequencing, generating data from >280 M clusters per lane): verification of quality and quantification in laboratory.-data analysis from genetic (Kdr-1014) and transcriptome data.-Moreover, I also support a Master student working with Environmental DNA.2)The project aim to ‘Dissecting the role of insecticide resistance in the spread of vector-borne disease’ and it was funded by Seed Award in Science, Wellcome Trust, UK (Grant reference number: 202137/Z/16/Z).-Select new sublineages of A. aegypti CAYMAN based on insecticide resistance profile by molecular screening to select kdr mutations in live mosquitoes. This task was complemented by bioecology experiments in insectary.-Select new sublineages of A. aegypti RECIFE based on insecticide resistance profile by WHO assays with papers treated with insecticides with different concentrations. This task was complemented by bioecology experiments in insectary.-Preparation of samples for RNA-sequencing from all lineages selected, heterozygous individuals from crossing and reference lineages.-Data analysis of de novo Transcriptomic data from all groups sequenced.-Support students and tecnical staff aiming to continue the project.

-I edited two manuscripts-I support other guest editors-I prepare an editorial in partnership with the other guest editors.EditorialGenta F. A., Diaz-Albiter H. M., Salgueiro P., & Gomes B. (2016) Control of Vector-Borne Human Parasitic Diseases. BioMed research international, 2016: p. 1-4

The study of the speciation process with gene flow among species of Anopheles gambiae complex in Guinea Bissau. Funded by Fundação para a Ciência e a Tecnologia, PT (FCT; PTDC/BIAEVF/120407/2010)-data analysis of the genetic information genotyped during the project (i.e. microsatellites, Kdr, ace-1 genes) that include clustering analysis (STRUCTURE: without geographic information, TESS: with geographic information) and infer effective population size in temporal populations;-Correlate ELISA identification of blood meals source of mosquitoes and KDR genotypes with clustering analysis and mosquito ID;-manuscript preparation;-support data analysis of PhD and Master students working with Culex pipiens and Glossina palpalis gambiensis.-Initiate the participation as collaborator of the project: PEst-OE/SAU/LA0018/2011 (CMDT, PT) - Patrícia Salgueiro (PI) - 2012 - 2013 /Past and present population genetics of the vector Aedes aegypti: a tool for dengue control policies. /The goal of this study was to determine the origin of the Aedes aegypti and their genetic characteristics regarding traits with impact to vector control (e.g. insecticide resistance).

Functional genomics of peptidases in sand flies and kissing bugs (both vectors of neglected tropical diseases). This project was funded by “Programa Ciência Sem Fronteiras” from the CNPq and CAPES, BR (400607/2012-3 – PDJ).-Participation into two genome annotation consortiums: 1) Triatomine model vector genome, Rhodnius prolixus; 2) two Sand flies genomes, Lutzomyia longipalpis and Phlebotomus papatasi.-Gene annotation of peptidases, gene orthology and protein structure inference that would be used in further biochemistry and molecular biology analysis.-Support data analysis of Master students and manuscript preparation.This project was a spinoff project from "Gene silencing mediated by RNA interference in larvae stage of insects with medical importance: new tools to study and manage genetic mechanisms", 400607/2012-3 (CNPq/CAPES, BR) - Rod Dillon (PI) - 01/07/2013 – 30/06/2014 /The goal of this study was to determine candidate genes associated with digestion to develop a control strategy based on RNAi.

Doctor of Philosophy (PhD) candidate at the Centro de Malária e Doenças Tropicais, Instituto de Higiene e Medicina Tropical, Universidade Nova de Lisboa (CMDT/IHMT), with a PhD work plan entitled “Genetic studies on the mosquito vector Culex pipiens”. The work plan was supervised by Prof. João Pinto (CMDT/IHMT), Prof. Paulo Almeida (UPMM/IHMT) and Prof. Martin Donnelly of the Liverpool School of Tropical Medicine (UK). The work plan was supported by a PhD fellowship of Fundação para a Ciência e a Tecnologia, Portugal (SFRH / BD / 36410 / 2007).Duties:-Field collection of mosquitoes from Culex pipiens complex in Portugal and UK.-Insectary experiments-Mosquito ID by molecular tools-Microsatellites analysis to carried population genetics-Optimization of an amplified fragment length polymorphism technique-Blood meal identification by ELISA-Correlate population genetics and ecology of Culex pipiens with West Nile virus epidemiology data.-Preparation of manuscriptOutcomes:- Thesis: Bruno Gomes da Silva (2013) "Genetic studies on the mosquito vector Culex pipiens".https://run.unl.pt/handle/10362/19277-2 awards:2014 Best doctoral student in Biomedical science, sub-domain Parasitology, in the “Prémio Professor Doutor João Fraga de Azevedo”, Instituto de Higiene e Medicina tropical, Universidade NOVA de Lisboa, Lisbon, Portugal.2011 Third classification in the “Oral presentation award” of the 6th European Mosquito Control Association workshop, Budapest, Hungary.- 6 publications associated with the thesis[1]Gomes B. et al. (2015) BMC Evolutionary Biology 15:197[2]Gomes B. et al. (2013) Infection Genetics and Evolution 16:218-225.[3]Gomes B. et al. (2013) Parasites & Vectors 6:93.[4]Gomes B. et al. (2012) Ecology and Evolution. 2(8):1889–1902.[5]Gomes B. et al. (2012) Journal of the American Mosquito Control Association, 28(4s), 75-80.[6]Gomes B. et al. (2009) BMC Evolutionary Biology, 9:262.

Coordinated the organisation of the EURODOC'13 - Europe’s Grand Societal Challenges: The role of Early Stage ResearchersTo further details consult http://lisbon2013.abic-online.org/

Events:-III Conferência do Emprego Científico e I feira de Emprego Científico-EURODOC'13 - Europe’s Grand Societal Challenges: The role of Early Stage ResearchersInternational affairs:-Coordinate the contacts with EURODOC (The European Council of Doctoral Candidates and junior researchers) and World Federation of scientific workers-Represent ABIC in International events- Official delegate in the Eurodoc (until 2011)General tasks of the Board (e.g. representation in meetings, preparation of policy papers)

- Coordinator of the Eurodoc's projects- Support of the Survey Working group (change to "Policy research" working group in 2012)- Responsible for the WG-Board meetings- Staff from the 1st European Summit For Early stage Researchers in Strasbourg (September)

Research Grant at Centro de Biologia Ambiental (CBA), in the project “Evolutionary processes in the origin of ‘hotspots for biodiversity’: insights from southern Portuguese areas based on novel nuclear multilocus approaches in target freshwater fishes and amphibians.” supervised by Prof.ª Maria M. Coelho (CBA). (6 months period).The project was supported by Fundação para a Ciência e a Tecnologia, Portugal (PTDC/BIA-BDE/69769/2006).-DNA and RNA extraction-Optimization of PCR od candidate genes in freshwater fishes-Collection of fish material for molecular analysis

Research Grant at the Centro de Malária e Doenças Tropicais, Instituto de Higiene e Medicina Tropical, Universidade Nova de Lisboa (CMDT/IHMT), in the project “Sistematics and Evolution of Culex pipiens in Portugal and Macaronesian Islands” supervised by Prof. João Pinto (CMDT/IHMT). (14 months period).The project was supported by Fundação para a Ciência e a Tecnologia, Portugal (POCI/BIA-BDE/57650/2004).- Entomological survey, field test of repellents in Comporta (Portugal) - Anopheles atroparvus and other Culicidae-Arbovirus entomological surveys in Alqueva (Portugal) focusing in Culicidae-Entomological surveys and insectary experiments in Madeira (Portugal) for Culex pipiens-Entomological surveys in Cape Verde for Culex pipiens and other Culicidae- Optimization of all molecular biology PCR.-Population genetics from Comporta- 1 publication[1]Alves J., Gomes B. et al. (2010). Mosquito fauna on the Cape Verde Islands (West Africa): an update on species distribution and a new finding. Journal of Vector Ecology, 35(2), 307-312.- BG obtained funded for PhD (succesful for 2 submission)2008 [Approved] Bruno Gomes successfully applies to a 4-year Fellowship for PhD project "Signatures of selection in insecticide target site loci", supervised by Martin Donnelly (LSTM, UK), and submitted to the Fundação para Ciência e Tecnologia (reference SFRH/BD/43545/2008). Approved by the biological sciences panel (I did not initiate this project due regulations).2007 [Approved after appeal] Bruno Gomes successfully applies to a 4-year Fellowship for PhD project "Genetic studies on the mosquito vector Culex pipiens", supervised by João Pinto (CMDT/IHMT, UNL, PT), and submitted to the Fundação para Ciência e Tecnologia (reference SFRH/BD/36410/2007). Approved on appeal by the biological sciences panel (assessment of 4.35 - maximum: 5).

Graduation Student at the Centro de Investigação em Biodiversidade e Recursos Genéticos, Universidade do Porto (CIBIO-UP), with a work plan entitled “Study of agouti gene polymorphism and two microsatellites in dog and wolf”. The work plan was supervised by Doutora Raquel Godinho. (6 months period).-Final project in genetics of dogs and wolves where I have my first experience with molecular biology and I optimize multiple PCR reaction, gel assays for the agouti gene by Single-strand conformation polymorphism, and two gel assays for microsatellites. -Optimize SSCP for gene agouti for dogs and wolves-Optimize gel analysis for two microsatellite (one marker had a strong variation between dog and wolves and it was defined as diagnostic- Final dissertation: Bruno Gomes da Silva (2006) Study of the agouti gene and two associated microsatellites in dogs and wolves. Final project for the Biology BSc. Hons, Faculdade de Ciências da Universidade do Porto, Portugal (funded by a 6-month scholarship POCI/N/70.01.02/0232/0030/2005).