Autoimmunity, Transplantation, Inflammation team (Dr Sylvain Perruche)Transplantation, Inflammation, Complications group (Pr Philippe Saas, Pr Didier Ducloux)under the supervision of Pr Philippe Saas and Dr Etienne DaguindauSubject : Acute Graft-versus-Host Disease (GvHD): impact of circulating lipopolysaccharides and role of the lipoprotein metabolism for their neutralization

Objectives- Providing care to animals (mice) (litter, food, water) and monitoring of sanitary status- Maintenance and cleaning of animal facilities- Support for team experiments* This position also allowed me to work on my research project between my Master 2 Internship and my PhD thesis.

Subject : Graft versus Host Disease (GvHD) - impact of intestinal permeability and bacterial translocationObjectives : Exploration of the impact of circulating LPS from intestinal microbiota and its potential neutralization by PLTP enzyme.- Bibliographic research- Validation of a mouse model of GvHD- In vivo and in vitro experiments, Data analysis- Redaction of an internship report and oral presentation- Evaluation of a new pro-resolutive drug (SuperMApo®) interest in GvHD mouse modelSkills and Techniques : In vivo experiment (mouse), GvHD assessment, Immunomagnetic cell sorting, Intestinal permeability assessment (Histopathology analysis, FITC-Dextran translocation test), ELISA test, Flow cytometry, GraphPad Prism, Development of experimental protocols

Subject : Liver X Receptors, a new therapeutic target for Blastic Plasmacytoid Dendritic Cells NeoplasmObjectives : Evaluation and qualification of the cell death induced by LXR agonists (GW3965 et T0901317) in CAL-1 BPDCN cell line. - Bibliographic research- In-vitro experiments and data analysis- Redaction of an internship report, oral and poster presentationSkills and Techniques : Cell culture, Flow cytometry, Real-time PCR, Western Blotting, GraphPad Prism, Development of experimental protocols