Chris Cacciatore Email & Phone Number

Boston, Ma, Us

Boston, Ma, Us

Lexington, Ma, Us

Lead verification studies by authoring comprehensive protocols, coordinating with cross-functional teams, performing analysis on data yielded, and authoring study reports. Collaborates with multiple departments including Quality Control and Regulatory Affairs to ensure study design and execution met project goals and regulatory requirements. Collaborates with Quality Control and Manufacturing teams through the design and execution of experiments to investigate root causes of failures observed in verification testing and develop effective corrective actions. Conduct sample preparation including DNA or RNA extraction, library preparation, and quality control measures to ensure optimal NGS sequencing performance in the detection of bacterial cells at titers ≥ 3 CFU/mL in a whole-blood matrix. Designs new quality control assays to evaluate polymerase enzyme and multiplex PCR buffer activity Develops and conducts troubleshooting experiments to aid in root-cause discovery for non-conforming manufactured reagents Trains newly hired associates to understand wet-lab and analytical technique while integrating them into the development process

Lexington, Ma, Us

 Lead verification studies by authoring comprehensive protocols, coordinating with cross-functional teams, performing analysis on data yielded, and authoring study reports. Collaborates with multiple departments including Quality Control and Regulatory Affairs to ensure study design and execution met project goals and regulatory requirements. Collaborates with Quality Control and Manufacturing teams through the design and execution of experiments to investigate root causes of failures observed in verification testing and develop effective corrective actions. Conduct sample preparation including DNA or RNA extraction, library preparation, and quality control measures to ensure optimal NGS sequencing performance in the detection of bacterial cells at titers ≥ 3 CFU/mL in a whole-blood matrix. Designs, evaluates and implements processes to remove/eliminate contaminating nucleic acids from reagents utilized in al diagnostic assays Trains newly hired associates to understand wet-lab and analytical technique while integrating them into the development process Employs analytical skills to offer input on development process direction by evaluating data and prior analysis produced by all members of assay group

Lexington, Ma, Us

 Independently designed, executed and analyzed both end-point and qPCR experiments to expedite assay time to result through development of an equivalently yielding two-step PCR protocol adapted from a nominal three-step process. Consolidated documented data into development reports written to educate management and grant donors on progress of development process. Evaluated viability of assay internal control candidates by monitoring competitive inhibition of panel target amplification, cross-reactivity with particle designed to detect other panel targets and its own amplicon yield

Venlo, Limburg, Nl

Designed and executed experiments to develop formulations and applications of assay mixes which incorporate novel enzymes produced by the protein development team  Independently analyzed qPCR and enzyme kinetic assay data to benchmark newly developed enzymes to understand the niche they would occupy in the market

Venlo, Limburg, Nl

Independently performed, document and analyze results of quality control assays for manufactured product release (enzyme product purity and specific activity determination, quantification of impurity/contamination levels) Proactively support continuous process improvement initiatives by developing, troubleshooting, revising and reviewing SOPs, qualification/validation protocols and reports for genomics reagents Trained new hires to quickly integrate them into the team’s core workflow

Venlo, Limburg, Nl

As a quality control technician, my core responsibilities lie in verification of enzyme concentrates and dilutions, reaction buffer and qPCR master mix quality. My other responsibilities are focused on supporting the product qualification process. I perform validations of quality control assays, produce substrates and buffers that are used in unit characterization and other assays, manage inventory and help to maintain the laboratory.While in this role, I provide continuous support to the production team by training and supervising new production technician and facilities employees.

Venlo, Limburg, Nl

-Wrote and performed equipment IQ/OQ/PQ protocols-Prepared buffers and diluents for retail sale-Operated and maintained a Sharples super centrifuge-Kept a detailed inventory of chemicals used in buffer and diluent prduction-Supported the purification team by preparing buffers and diluents for manufacturing-Supported all laboratories by cleaning and sterilizing labware-Performed preventative maintenance and calibrations on laboratory equipment and instruments

Beverly, Massachusetts, Us

-Prepared media and poured plates for use in microbiology classes-Prepped starter bacteria cultures for microbiology classes-Prepared solutions for use in cheimisty classes-Maintained all laboratory areas-Disinfected and autoclaved soiled glass and plastics-Maintained laboratory equipment (microscopes, hoods, pipettes)-Kept inventory of chemical stocks

Beverly, Massachusetts, Us

Aided my professor in his research. -Performed RNA extractions from skate tissue-Designed primers for genes of interest in the skate-performed end-point RT-PCR to evaluate gene expression-Performed intraperitnneal injections on skate specimens-Sacrificed skate specimens and harvested tissue for RNA extraction

Boston, Ma, Us

A semester-long internship-Tranfected immature human B-cells with a modified cell-surface protein-Selectively cultured cells to achieve stable transfection-Performed RNA extraction and subsequent purification for use in evaluation of gene expression-Designed primers for highly repetitive DNA sequence for evaluating gene expression-Performed reverse transcription and end-point PCR on purified RNA extractions to evaluuate gene expression-Performed viral binding assays

Salisbury Cove, Me, Us

Advised students on laboratory techniques, theory and procedures pertaining to the elucidation of mRNA in the species Leucoraja erinacea (the Little Skate). Students were participants in the Maine IDeA Network for Biotechnological Excellence (INBRE) program, who were given grants to pursue research with a PI assigned to them.

-Prepared lectures on topics ranging from biology to astronomy for students enrolled in the Lynn Learning Leadership program -Responsible for the well-being and behavior of 13 - 14 year olds in a dorm environment.-Was an active leader in extracurricular activities (sports and recreation).