Christopher B. Email & Phone Number

Ipswich, Ma

Production Scientist in the Competent Cell production group at New England Biolabs (Ipswich).

Ipswich, Massachusetts

-Production of competent cells (various cloning and expression strains) for sale in the research market.-Provide technical support for customers.-Maintain weekly and monthly stock records of competent cell products.-Perform transformations and quality control to provide exceptional competent cells to customers.-Buffer and media preparation.-Assist with packaging of products and complete bulk orders.

Perform In-Process microbiology testing, including bioburden, endotoxin (kinetic chromogenic, turbidimetric, gel-clot), TOC analysis, and other USP and EP testing requirements to ensure that the product is safe and free of microbial contamination.Validation of Sievers M9 TOC analyzers and peristaltic pumps for dispensing final product stability samples, including creating new GMP documents for their use and revising GMP documents.Piloting assay forms on the Perkin Elmer iLAB electronic worksheet system and offering improvement ideas for ease of use.General lab housekeeping and stocking.

• Perform Mouse Embryo Assays (MEA) on IVF devices, media, and oil.• Culture mouse embryos and record stages of embryo development daily.• Test devices, media, and culture oil for presence of endotoxin using both the Gel-Clot and Turbidimetric Limulus Amoebocyte Lysate (LAL) assay.• Use of Good Documentation Practices (GDP) to create Certificates of Analysis.

• Assisted with research on various innate immune system projects, including work on scavenger receptors on macrophage cells.• Prepared mice splenocytes for flow cytometric analysis.• Performed immunomagnetic isolation from mice spleens and stain cells with CFSE dye.• Carried out genomic DNA isolation and PCR to verify the genotype of knock-out mice.• Co-author of abstract: SCARF1 mediates apoptotic cell clearance and autoimmunity.

Bachelor Of Science (Bs), Biology, General

Education record