Christopher Bianchetti Email & Phone Number

Middleton, Wi, Us

Middleton, Wisconsin, United States

Middleton, Wisconsin, United States

Madison, Wisconsin Area

Department of Chemistry● Designed and implemented the cloning, expression, purification, and enzymatic characterization of 32 putative quorum quenching enzymes ● Led an independent research laboratory and mentored 15 undergraduate research students ● Designed high throughput PCR based cloning and automated protein purification protocols for undergraduate researchers in my laboratory

Madison, Wisconsin Area

Advisor: Prof. Brian G. Fox● As part of a team of Great Lakes Bioenergy Center researchers I utilized bioinformatics, biochemistry and X-ray crystallography to elucidate the structural features that are unique to multifunctional biomass-degrading enzymes● Utilized high-throughput in vitro cell-free protein synthesis to express and characterize >100 biomass-degrading enzymes ● Determined the structure and biochemical properties of a novel secreted caffeoyl-CoA dioxygenase, which may enhance invasive attack on plant biomass by preventing lignin formation● Determined structures and functional properties of four previously uncharacterized enzymes from the highly celluolytic bacteria Streptomyces sp. SirexAA-E that are upregulated when grown on lignocellulosic biomass

Madison, Wisconsin Area

Advisor: Prof. George N. Phillips, Jr.● As part of the Center of Eukaryotic Structural Genomic team I utilized high throughput methods to purify, crystallize and determine the three dimensional structures of multiple proteins (13 PDB entries) ● Utilized X-ray crystallography, SAXS, and enzymatic assays to characterize the interactions between key aromatic residues that line the cellulose-binding cleft of EngD and soluble cellulose fragments● Utilized X-ray powder diffraction to determine cellulose crystallinity and underlying substructure and correlated cellulose structure with enzymatic digestibility● Determined the structure, the nitric oxide binding properties, and proposed a probable function for a previously uncharacterized heme-containing enzyme nitorbindin

The Center For Advanced Microstructures And Devices

Advisor: Prof. Amitava Roy ● Provided data collection, processing, and analysis support for users of the X-Ray absorption spectroscopy beamlines at CAMD ● Responsible for the construction and maintenance of the X-ray powder diffraction beamline at CAMD● Characterized the absorption of chromium and arsenic into plant tissue using X-ray absorption spectroscopy and high-resolution X-ray tomography

Doctor Of Philosophy (Phd), Biophysics

Activities and Societies: Advisor: Prof. George N. Phillips, Jr. Thesis: Structure And Functional Characterization Of Cellulases And Their.

Bachelor Of Science (B.S.), Microbiology

Minor in Chemistry