Christopher B. Email & Phone Number

Ashburn, Virginia, United States

Bethesda

The National Heart, Lung, and Blood Institute (NHLBI) provides global leadership for a research, training, and education program to promote the prevention and treatment of heart, lung, and blood diseases. The NHLBI stimulates basic discoveries about the causes of disease, enables the translation of basic discoveries into clinical practice, fosters training and mentoring of emerging scientists and physicians, and communicates research advances to the public.

Basel

I've developed a correlative light and EM (CLEM) technique at C-CINA, Basel, Switzerland and employ a stage onto which I can load ultrathin samples attached to EM grids to visualize them directly in the fluorescent microscope. I can then map areas of interest, say on GFP labeled proteins and then load the same grids into the transmission electron microscope for high resolution imaging and/or electron tomography. This allows to see spatial distributions and the inter-relationships between various cellular components.I am currently applying this technique in a variety of biological problems.Additionally, I am studying Salmonella that cause diarrhea or a more life-threatening systemic disease called typhoid fever. During typhoid fever, spleen and liver are appointed to act as major target organs, in which Salmonella reduplicate mostly intra-cellularly in phagosomes of infected macrophages. Cell culture infection studies propose that some Salmonella escape from the phagosomes to the host cell cytosol where they can be killed in a process involving host cell degradation (autophagy/xenophagy). However, the frequency of such events in vivo still remains unclear.Therefore, I am using the unique capabilities of the serial block-face scanning electron microscopy (SBF-SEM) technique to investigate host / Salmonella interactions simultaneously at the tissue, cellular level in a mouse typhoid fever model. Such large 3 dimensional data sets will provide a unique basis for unprecedented comprehensive analysis of host-pathogen in vivo interactions.

Basel Area, Switzerland

In my Ph.D. project, I studied the entry pathway of the Vaccinia virus with high-resolution cryo-electron microscopy. We described a new macropinocytic mechanism of extracellular enveloped virus and I worked on the Vaccinia virus early core activation and uncoating.

Zürich Area, Switzerland

Basel Area, Switzerland

Osaka, Osaka, Japan

Heidelberg Area, Germany

Heidelberg Area, Germany

Heidelberg Area, Germany

Heidelberg Area, Germany

Part time

Doctor Of Philosophy (Phd), Structural Biology, Summa Cum Laude

Master Of Science (Ms), Biology/Biological Sciences, General

Bachelor'S Degree, Biology/Biological Sciences, General

Technical High School, Higher Education/Higher Education Administration

Research Technician, Biology/Biological Sciences, General

Secondary School, Secondary Education And Teaching