Christopher Rex Email & Phone Number

Graham, Nc, Us

Durham, North Carolina, United States

Provided math tutoring services to students in grades 4-12, including ACT/SAT test prep.

Mebane, North Carolina, United States

-Taught honors and general Biology courses for grade 9-12 students-Was awarded a $10,030 Impact Alamance grant for Biology course materials/equipment (3/2024)-Crafted questions for the NC HS Biology EOC and 8th Grade Math EOG tests-Worked on the District Science Implementation Team to help teachers adapt their curricula to the new EOC Science standards-Assisted in running the Science Olympiad and Dungeons&Dragons (DnD) Clubs-Tutored students during Evening Academy (after school) and Saturday School in Science and Math

Wake Forest, North Carolina, United States

-Served as remote data analyst for the Toxicology (CLIA-certified) lab-Used Analyst software to analyze LC/MS data and determine the presence/absence/abundance of various drugs during toxicology screening of human urine or saliva samples-Ensured accuracy of patient results and demographic information prior to sending reports to clinics-Developed novel drug/clinic lists, optimized the analyte retention time document, and crafted training protocols for performing various aspects of the job-Continued working with the LC/MS machine team (from previous Radeas position) to help manage the machines and ensure the collection of quality data

Wake Forest, North Carolina, United States

-Served as third shift LC/MS operator for the Machine Team (Toxicology) in a CLIA lab-Used LC/MS to determine the presence/absence of various drugs for the toxicology screening of human urine or saliva samples-Prepared solutions/buffers/QCs and batched/submitted sample runs with Analyst software on the LC/MS-Ensured the fidelity of batched samples (correcting them as necessary), the migration of data files to a networked drive, and the adherence to sample reporting deadlines-Performed daily logging of refrigerator/room temperatures (logging corrective measures taken), daily running of QCs, and weekly cleanings and STDs for the machines-Worked to collect quality data on the LC/MS machines by taking them apart (curtain plate, exhaust, stator, orifice plate, skimmer) and cleaning them (using soap, isopropanol, methanol, or sonication)-Changed out buffers, peek tubing, fittings, columns, column filters, and performed Q0 and MS rail cleanings-Analyzed sample data for the detection of cocaine according to preset threshold values after selecting/trimming peaks appropriately using Analyst software-Acted as lone liaison between personnel at the end of second shift and the beginning of first shift in order to adequately communicate sample run progress, datafile locations, machine maintenance performed, and sample movements completed-Engineered a 3-D printed solution to resolve the frequent needle slip faults we were getting on the HPLCs by crafting a stabilizing plastic PLA+ tube sleeve to cradle/straighten our 1.5 ml conical microcentrifuge tubes on the 4500 tube racks (reducing these faults to near-zero)-Developed novel machine maps, cards for information about QC standards, and protocols for performing various aspects of the job-Specific LC/MS machines used include the Shimadzu Nexera series high pressure liquid chromatography (HPLC) and AB Sciex 3200 and 4500 mass spectrometers (MS/MS)-Continued working with the COVID-19 lab (from previous Radeas position)

Greensboro, North Carolina, United States

CRISPR Development of an Epigenome Editing Tool to Correct Environmentally-induced Epigenetic Mutations-Crafted a clustered regularly interspaced short palindromic repeat (CRISPR) technique for removing or adding methylation to the DNA of Medaka fish (Oryzias latipes)-Designed oligos and primers to capture and recruit the catalytic domain of various methylating or de-methylating enzymes (DNMT3A, TET1, etc.) from Medaka cDNA-Used a variety of cellular/molecular techniques such as primer design, plasmid vector design/use, PCR, 1-D SDS-PAGE, and microinjection of living fish embryos-Helped maintain a Medaka fish colony by feeding fish, cleaning tanks, and raising fish from eggs-Taught labs for Biology 111 (Principles of Biology 1) and Biology 277 (Human Physiology)-Taught pre-organized labs that required the assembly of PowerPoints to introduce the material-Performed all grading and class supervision, helping students with questions and troubleshooting the lab as problems came up

Wake Forest, North Carolina, United States

-Served as third shift team lead for the COVID-19 PCR lab (Molecular Diagnostics)-Processed patient nasal/saliva samples in order to diagnose the presence of the SARS-CoV-2 virus-Transferred, accessioned, extracted, and eluted samples to run on high-throughput RT-PCR-Manually analyzed samples using COVID-19 Interpretive Software for the presence of 3 viral genes-Crafted and optimized protocols for every step, including the creation of positive controls, to improve time efficiency and COVID-19 test efficacy while reducing rerun rates-Utilized a variety of pipettes and other laboratory equipment, including the KingFisher Flex and QuantStudio 5 RT-PCR machine-Relied upon LC/MS to determine the presence/absence of various drugs for the toxicology screening of human urine or saliva samples

Henderson, North Carolina, United States

Taught lecture and lab for Anatomy & Physiology 1&2 classes and lecture for Pathophysiologyclass (both online and in-person formats).

Durham, North Carolina, United States

Provided team math tutoring services to students in grades 4-12.

San Diego, California, United States

Taught one 3-week session of Through the Microscope (SCOP) to gifted third/fourth graders.

Fort Wayne, Indiana Area

Taught lecture and lab for Anatomy & Physiology 101 & 102 (A&P 1 and 2) and Biology 100(Human Biology) & 101 (Introductory Biology) classes.

Muncie, Indiana Area

-Taught lecture and lab for Zoology 330 (Comparative Anatomy) and Biology 111 (IntroductoryBiology) classes-Served on the Student-Alumni Relations committee and helped coordinate/setup events

Fort Wayne, Indiana Area

High School Biology/Math Teacher at Fort Wayne Community Schools

Fort Wayne, Indiana Area

-Managed teams of people and coordinated special events-Trained new staff and monitored their performance-Assembled/optimized protocols and shift duties for staff-Tracked inventory and maintained ~850 items in the computer system-Produced monthly stock/retail value and loss reports for all inventory items-Created food/drink menus and event flyers-Performed various maintenance tasks to optimize operation of the shop-Balanced cash register and transported cash deposits to the bank-Served cigars, wines, beers, chocolates, and food to patrons

Columbia City, Indiana

Substituted for a variety of middle/high school classes.

Greeley, Co

Worked on two Master's projects under Dr. Stephen Mackessy: "Effects of Captivity on Western Diamondback Rattlesnake (Crotalus atrox) Venom Composition and Effects of Season on Prairie Rattlesnake (Crotalus viridis viridis) Venom Composition"-Performed 1-D SDS-PAGE, a variety of enzyme assays (utilizing spectrophotometric analysis), protein concentration assays, RP-HPLC, size exclusion, LD50 (i.p. in mice/lizards), data analysis (using Minitab software)-Coordinated and directed a team of people in performing rear-fanged snake venom extractions by first giving s.c. injections of ketamine and pilocarpine to snakes and then placing capillary tubes over the snake’s fangs to collect venom-Worked as part of a team assisting with front-fanged snake venom extractions, including placing capillary tubes over the snake’s fangs to collect venom and PIT-tagging them-Presented results in Powerpoint format for three Springs (2009, 2010, 2011), plus three conferences: at the 17th World Congress of the IST and Venom Week 2012 joint-conference in Honolulu, HI on July 11, 2012; at the Biology of Rattlesnakes symposium in Tucson, AZ on July 22, 2011; at a SouthWest Partners in Amphibian and Reptile Conservation meeting in Fort Collins, CO on August 5, 2010.-Managed a team of people in performing weekly husbandry duties (feeding, cleaning, etc.) for ~120 snakes (primarily venomous) and lizards (including venomous)-Periodically assisted in the capture of Prairie Rattlesnakes from two local den sites

Greeley, Co

-Executed both labs that were pre-ordained and labs that required setup, lesson plan design, and the assembly of Powerpoints-Performed all grading and class supervision, helping students with questions and troubleshooting the lab as problems came up-Taught Herpetology, Mammalogy, Parasitology, Bio 110, and Anatomy/Physiology labs

Muncie, In

Worked on a project under Dr. Michael Litt: "Knocking Down USF-1 to Study Epigenetic Modifications Across the Chicken β-globin Locus During Erythroporesis"-Examined how an insulator modulated histone modifications during RBC development-Created a USF-1 knockdown in 6C2 (chicken blood) cells and examined histone modifications in those cells-Used a variety of cellular/molecular techniques such as RNAi, plasmid vector design/use, cell culture, miniprep of plasmids, nuclear extracts, 1-D SDS-PAGE, western blotting, and transfection of bacterial and avian cells-Presented results in the form of a poster and Powerpoint presentation for each of the three summers in the presence of fellow Chemistry students and faculty

Master Of Science, Biological Sciences, 3.61/4.00

Activities and Societies: Fencing ClubDegree with thesis option, 55 total credits.

Doctor Of Philosophy - Phd, Environmental Health Science, 3.8

14 total credits. Left prior to graduating.

Associate’S Degree, Computer Software Engineering, 3.9

30 total credits. Left prior to graduating.

Bachelor Of Science, Zoology, Ecology, Biochemistry, 3.52/4.00

Activities and Societies: Fencing Club141 total credits.