Lead the formulation development of therapeutic protein

Formulation development and stability program of therapeutic protein

In Glyconex, I am mainly responsible for setting up new technology platforms for antibody generation and characterization. The applications of the platforms include glycan derivatization, high-throughput specificity screening, affinity measurement by Biacore, N-glycan characterization by UPLC and glycan purification by HPLC. I also design and lead the training program for junior associate researcher in Glyconex. As a trained protein crystallographer in Glyconex, I prepared antibody candidate… Show more In Glyconex, I am mainly responsible for setting up new technology platforms for antibody generation and characterization. The applications of the platforms include glycan derivatization, high-throughput specificity screening, affinity measurement by Biacore, N-glycan characterization by UPLC and glycan purification by HPLC. I also design and lead the training program for junior associate researcher in Glyconex. As a trained protein crystallographer in Glyconex, I prepared antibody candidate for crystallization and organized the cooperation with scientists of National Research Program for Biopharmaceutical Show less

I was interested in the catalytic mechanism of a unique enzyme called POFUT2. I used tools such as X-ray crystallography and mass spectrometry to investigate the structural and functional relationship of POFU2. The skills I have use in this project include:- Cloning, expression, purification of glycoprotein - Characterization of glycans on the glycoprotein by mass spectrometry- Protein expression and optimization in mammalian & prokaryotic systems- Optimization of protein… Show more I was interested in the catalytic mechanism of a unique enzyme called POFUT2. I used tools such as X-ray crystallography and mass spectrometry to investigate the structural and functional relationship of POFU2. The skills I have use in this project include:- Cloning, expression, purification of glycoprotein - Characterization of glycans on the glycoprotein by mass spectrometry- Protein expression and optimization in mammalian & prokaryotic systems- Optimization of protein purification via various chromatography techniques - Protein crystallography - Enzyme kinetic by HPLC-MS- Protein engineering Show less