Connor Ludwig Email & Phone Number

Emeryville, California, United States

Developing multiplexed, cell-based screening approaches to expand Octant's drug discovery capabilities

Department Of Bioengineering - Bintu Lab

Performed and developed high-throughput sequencing-based assays to measure gene regulatory and immunomodulatory functions of viral proteins

Institute For Neurodegenerative Diseases - Kampmann Lab

Established CRISPR interference and activation technologies in iPS cell-derived neurons to conduct genetic screens focused on elucidating pathways in neurodegenerationExplored the interplay between amyloid beta and tau pathology in human cellular models of Alzheimer's diseaseDeveloped computational pipelines in ImageJ and R to simplify, automate, and streamline quantification of large, image-based data sets

Ucsf - Green Lab; Gladstone Institute Of Neurological Disease - Gan Lab

Utilized in silico plasmid design, molecular cloning, induced pluripotent stem cells, and cutting edge proteomic techniques to better understand the underlying mechanisms of disease and discover novel players in frontotemporal dementia.Regularly performed PCR, cloning, bacterial and mammalian cell culture (lymphoblasts, fibroblasts, iPSCs, primary and derived neurons, and astrocytes), transfection and transduction, immunofluorescence staining, microscopy, high-throughput imaging, flow cytometry, and protein purification for affinity-purification mass spectrometry.Actively collaborated with and consulted world experts in neurology, ophthalmology, and biochemistry at UCSF.Utilized engineering design to improve, simplify, and economize laboratory practices, techniques, and assays.

Department Of Bioengineering - Healy Lab

Contributed to the optimization of differentiation and culture conditions of iPSC-derived endothelial cells for downstream three-dimensional co-culture studies with cardiomyocytes for myocardium tissue regeneration following a myocardial infarctionWorked with four other undergraduate students and a supervising graduate student to develop an HIV CD4+ T cell enumeration device using magnetic cell sortingPresented experimental findings and relevant background research to the group and additional interested parties on a weekly basisMeticulously maintained a detailed, organized, and comprehensive lab notebook documenting experimental results, lab protocols, and other important informationExtensive experience in experimental design, endothelial and iPS cell culture, bright-field, fluorescence, and confocal microscopy, immunostaining, flow cytometry, and data analysis on Kaluza and Prism

Department Of Pathology - Abbas Lab

Performed immunological research aimed at identifying cellular and molecular mechanisms responsible for establishment and maintenance of tolerance to self-antigen, with a focus on mechanisms for CD4+ T cell-mediated autoimmune diseasesPhenotypically characterized cutaneous leukocyte sub-populations in BALB/c and B6 mice Optimized murine skin digestion protocol for improved cell characterization studies and anti-GATA-3 staining for flow cytometryExtensive experience with PCR, gel electrophoresis, flow cytometry, and analyzing data on FlowJoIndependently conducted the following on a regular basis: blood genotyping and organ harvesting/processing (skin, lymph nodes, spleen, marrow)

Department Of Pathology - Abbas Lab

Assisted fellow researchers in immunological research aimed at identifying cellular and molecular mechanisms responsible for establishment and maintenance of tolerance to self-antigen, with a focus on mechanisms for CD4+ T cell-mediated autoimmune diseasesExtensive experience with PCR, gel electrophoresis, flow cytometry, and analyzing data on FlowJo

Doctor Of Philosophy - Phd, Bioengineering And Biomedical Engineering

Activities and Societies: BioE Graduate Student Association

Master Of Science - Ms, Bioengineering, 4.07

Bachelor Of Science - Bs, Bioengineering, 3.89

Activities and Societies: Biomedical Engineering Society, Berkeley Engineering Initiative, Innovative Design, DeCadence A Cappella.

High School Diploma

Activities and Societies: Campolindo Swimming, Chamber Choir, Concert Choir, Boy Scouts