Cécile Mathieu Email & Phone Number

Cambridge, Massachusetts, United States

Cambridge, Massachusetts, United States

Cambridge, Massachusetts, United States

- Designed and implemented workflows to investigate the mechanisms of condensation for novel target discovery: cell-based characterization of protein mutants propensity to phase separate and interact with key protein or nucleic acid partners, using overexpression, ligand and light-induced system. - Integrated biochemistry, biophysics, and cell biology approaches to characterize small molecule mechanism of actions and interrogated their effect on condensate formation and signaling pathways: designed and implemented purified protein and lysate-based LLPS imaging assays, established phase diagrams in vitro and in cells, monitored downstream signaling markers using various approaches including in-cell western - Worked cross-functionally as a core team member with drug discovery, biology, and imaging teams to achieve project goals- Led collaborations with external partners and managed CROs- Managed one direct report by providing timely feedback on strength and weaknesses and guidance on career development. Ensured daily tasks prioritization to meet company goalsSkills:• Biochemical aproaches: protein-protein or protein-ligand interactions (DLS, Fluorescence polarization, FRET), phase separation assays, pull down/immunoprecipitation assays, western blot, in-cell western• Cellular biology: Cell culture, immunostaining, FISH, high-content imaging• Fluorescence microscopy: DIC, widefield, confocal, fixed and live-imaging, FRAP• Image analysis: Fiji, Gen5

Memphis Tennessee

- Elucidated the molecular and cellular mechanisms underlying the formation and regulation of RNA-protein condensates using biochemical and cellular tools. - Developed cellular tools and imaging workflow for the study and manipulation of liquid-like condensates:• Design and optimization of cellular biosensors for the detection of protein conformations• Engineering of proteins allowing the tunable formation of liquid-like condensates • Development of an imaging and image analysis workflow to map protein phase diagrams- Published 2 first author peer-reviewed publications - Presentation of findings in several national and international meetings- Trained and oversaw the work of staffs and graduate students- Multidisciplinary collaborations with 2 research groups at St. Jude Children’s research hospital to conduct advanced structural analyses of proteinsSkills:• Cloning and production of folded and disordered proteins• Biochemical aproaches: RNA-binding, protein-protein or protein-ligand interactions, phase separation assays, pull down assays• Structural biology: Structural analyses, experience with NMR and CryoEM• Cellular biology: Cell culture, immunostaining, FISH• Fluorescence microscopy: DIC, widefield, confocal, fixed and live-imaging • Image analysis: Cell profiler, ilastik, FIJI, Igor Pro

Paris

- Elucidated the structure-function relationship of metabolic enzymes: • Developed and optimized protein production and enzymatic assays• Crystalized and analyzed novel structures- Identified and characterized novel regulatory mechanisms of enzymes - Described new molecular mechanisms underlying the toxicity of Parkinson’s disease-associated pesticides: • Performed small-scale chemical screens, assessing their impact on enzymatic activities of target proteins• Characterized the mechanism of action using biochemistry and structural biology- Published 5 first author peer-reviewed publications - Presented findings in several national and international meetings- Collaborated with 2 research groups at Pasteur Institute (Paris, France) as part of additional structural and biophysical characterizations of enzymes- Supervised 2 undergraduate and high school students- Managed lab suppliesSkills:• Cloning and protein purification• Biochemical aproaches: enzymatic assay, enzymatic inhibitions, redox potential measurements, protein-ligand interactions, SDS-PAGE, Western Blot• Cellular Biology: Cell culture • Structural biology: protein crystallization, structural analyses

Paris, France

Introductory classes to biochemistry and enzymology at college level (40 students divided in 2.5 groups; 64h/year)

Gif Sur Yvette

- Investigated regulatory mechanisms of actin polymerization by developing expression and production protocols of potential protein regulators and assessing their role in reconstituted polymerization assay in vitro.- Co-author on 1 peer-reviewed publication

Doctor Of Philosophy (Phd), Biochemistry, Biomolecules And Therapeutic, Félicitations Du Jury

Structure and Regulation of Brain Glycogen Phosphorylase

Master'S Degree, Biochemistry, Cells, Therapeutic Targets - Pathological And Therapeutic Macromolecules, Grade : 3Rd

Master'S Degree, Biochemistry, Cells, Therapeutics Targets - Minors: Drug Design And Business Economics, Grade : 1St

Licence Degree, Biochemistry And Molecular Biology - Business Economics, Grade : 1St

Activities and Societies: As part of my degree, I was asked by the company Sita, based next to Paris, to evaluate its needs and spending.