Daniel Gitterman Email & Phone Number

Cambridge, United Kingdom

Cambridge, United Kingdom

- CRISPR-based gene targeting producing landing pad cell lines. - Vector design and construction, cloning strategies, site-directed mutagenesis, Golden Gate cloning, recombineering

Cambridge

- line management, training and mentoring of other team members - all aspects of cell line generation process and genotypic validation - various aspects of project management (proposals, feasibility, identifying risk factors, ensuring lab work completed to timelines, trouble shooting, interim and final reports)- client contact (regular update teleconferences, handling of ad hoc queries from before project initiation through to after completion)- All aspects of cell culture: plasmid transfection, viral infection, cell line characterisation, maintenance of cultures, automation using liquid handling robots, clonal isolation, feeder layers, cryo-preservation and revival of cell lines, management of liquid nitrogen cell banks- vector design, primer design, virus preparation, gDNA/RNA extraction, PCR (from large scale screens to specific genotyping), sequence analysis, qPCR, droplet digital PCR, Western blots- Flow cytometry and clonal isolation using FACS Aria III

Cambridge / Birmingham

- all five foundation level examinations passed· drafting patent applications before UK-IPO and EPO· ongoing prosecution of patent applications in numerous territories incl. EPO, USA, Japan, Australia· patentability and freedom-to-operate opinions for medical and mechanical devices· invalidity and infringement opinion for a mechanical devicesI was one of nine trainee patent attorneys that Barker Brettell made redundant in November 2009

Cambridge

Assisted electrophysiology team in characterising the interactions of new chemical entities with target ion channels.

Cambridge, United Kingdom

- characterising interaction of new chemical entities with target ion channels as part of multi- disciplinary team - providing electrophysiological support to other research programs e.g. safety pharmacology assays for lead compounds - literature reviews with a view to identifying possible new therapeutic targets - patch clamp recordings of voltage and ligand-gated ion channels in primary DRG neurons- dissection, preparation and maintenance of primary DRG neurons and cardiac myocytes, maintenance of recombinant cell lines

Harlow

- Designing and conducting experiments to characterise electrophysiological effect of stretch injury on brain tissue- regular updates and exchange of ideas with collaborating groups within EU framework grant- dissection, preparation and maintenance of organotypic hippocampal cultures, extracellular recordings using microelectrode arrays, cellular viability assays

Phd, Cell Physiology

Ba (Hons), Biochemistry, 2:1