Daniel Mchugh Email & Phone Number

San Diego, California, United States

San Diego, California, United States

• Worked as a member of the Core Biology team
• Investigated CRISPRi targeting of lncRNAs in Hepatic Stellate Cells (HSCs)
• Engineered cell lines to express therapeutic targets using lentiviral transduction and Jump-In HEK293 lines
• Developed an antibody-siRNA conjugate gene knockdown assay with a qPCR readout
• Used robotic automation to adapt qPCR-based KD assay to a high-throughput format
• Screened >1500 antibody-siRNA conjugates against multiple targets, driving advancement of high priority conjugates to in vivo examination

Cleveland, Ohio, United States

• Evaluated ~1000 compounds for their capacity to induce differentiation of primary murine oligodendrocyte progenitor cells into mature oligodendrocytes using a dose-responsive, cellbased functional assay
• Prepared semithin and ultrathin sections of the mouse spinal cord and optic nerve for light and electron microscopy over 15+ in vivo efficacy studies
• Programmed numerous ImageJ macros to improve throughput and accuracy of SEM image analysis
• Spearheaded the development of a oligocortical spheroid assay to examine compound-indcued differentiation of human oligodendrocyte progenitor cells
• Combined R, ImageJ, and Excel VBA code to build automated pipelines for data processing on multiple projects
• Analyzed data, prepared presentations, and reported results to internal and external project members

Cleveland/Akron, Ohio Area

Cleveland/Akron, Ohio Area

I worked in the Lab of Dr. Craig Hodges, a leading expert in Cystic Fibrosis animal modeling. My research involved examining correction of Cystic Fibrosis manifestations in the intestine. I also worked to identify novel therapies for Cystic Fibrosis which has been caused by nonsense mutations using high-throughput screening. In the Hodges Lab, I carried.

Villanova, Pennsylvania

I worked as a teacher's assistant for Human Anatomy I and II, as well as Biology for Non-Majors. I performed pre-laboratory lectures, and led students through various experiments, including rat dissections, blood typing, and histology.

Education record

Master Of Science - Ms, Biology

I worked in the lab of Dr. Matthew Youngman, studying the relationship between the FOXO transcription factor DAF-16 and SMK-1, a nuclear.

Bachelor Of Science - Bs, Biology