Summer Internship
During my internship at the PGJCCR Remote Summer Research Programme, I worked alongside GenoME Diagnostics. This involved continuing previous research from my GenoME Diagnostics summer internship in June 2022 into the identification and validation of blood-based early detection cancer biomarkers. I was required to work with other laboratory members to test the expression of previously researched biomarkers from our list to evaluate their specificity to Breast, Ovarian and Pancreatic cancers. Furthermore, we designed primers and optimised their specificity to our biomarkers through analysing their optimum annealing temperatures. I was tasked with maintaining the health of our patient cells in tissue culture through utilising aseptic technique and extracting patient DNA for use in our experiments.From these results I produced a full study with the aim of identifying blood-based DNAme biomarkers to potentially produce a reliable and accurate test for early diagnosis of High-Grade Serous Carcinoma. Then I presented my findings to the staff members of the Patrick G. Johnson Centre for Cancer Research.